Comparison of fecal coliform agar and violet red bile lactose agar for fecal coliform enumeration in foods.

Applied and Environmental Microbiology
A LeclercqP Baylac

Abstract

A 24-h direct plating method for fecal coliform enumeration with a resuscitation step (preincubation for 2 h at 37 +/- 1 degrees C and transfer to 44 +/- 1 degrees C for 22 h) using fecal coliform agar (FCA) was compared with the 24-h standardized violet red bile lactose agar (VRBL) method. FCA and VRBL have equivalent specificities and sensitivities, except for lactose-positive non-fecal coliforms such as Hafnia alvei, which could form typical colonies on FCA and VRBL. Recovery of cold-stressed Escherichia coli in mashed potatoes on FCA was about 1 log unit lower than that with VRBL. When the FCA method was compared with standard VRBL for enumeration of fecal coliforms, based on counting carried out on 170 different food samples, results were not significantly different (P > 0.05). Based on 203 typical identified colonies selected as found on VRBL and FCA, the latter medium appears to allow the enumeration of more true fecal coliforms and has higher performance in certain ways (specificity, sensitivity, and negative and positive predictive values) than VRBL. Most colonies clearly identified on both media were E. coli and H. alvei, a non-fecal coliform. Therefore, the replacement of fecal coliform enumeration by E. coli enumera...Continue Reading

References

Aug 1, 1979·Applied and Environmental Microbiology·D J ReasonerE E Geldreich
Sep 1, 1988·Annales De L'Institut Pasteur. Microbiology·A BéjiH Leclerc
Mar 1, 1974·The Journal of Applied Bacteriology·A W Hoadley, C M Cheng
Jun 1, 1974·Applied Microbiology·D W FrancisR M Twedt
Aug 1, 1984·Applied and Environmental Microbiology·B J Robison
Aug 20, 1989·Biotechnology and Bioengineering·J V StraightN B Jansen
Feb 1, 1982·Journal of Food Protection·R N Alexander, R T Marshall
Mar 1, 1983·Journal of Food Protection·K F WeissS Malcolm

❮ Previous
Next ❯

Citations

Sep 18, 2010·Plant Foods for Human Nutrition·Isabel SospedraJordi Mañes
Jan 16, 2004·Lancet·M Chantal KandhaiMike van Schothorst
Mar 3, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D DrudyS Fanning
Jun 30, 2007·Foodborne Pathogens and Disease·Edith Torres-ChavollaRosalba Gutiérrez-Rojo
Oct 14, 2009·Foodborne Pathogens and Disease·Chia-Hsiang ChangCheng-Chun Chou
Sep 8, 2006·Applied and Environmental Microbiology·Hoikyung KimLarry R Beuchat
Jan 19, 2006·Clinical Microbiology Reviews·J Michael Janda, Sharon L Abbott
Apr 7, 2009·International Journal of Food Microbiology·Larry R BeuchatGlenner M Richards
Mar 3, 2007·International Journal of Food Microbiology·Miriam Friedemann
Oct 7, 2004·Journal of Biotechnology·Yasunori TanjiHajime Unno
Jan 24, 2009·Wei sheng wu xue bao = Acta microbiologica Sinica·Guiming ZhaoYing Chen
Jul 26, 2005·International Journal of Food Microbiology·Joshua B GurtlerLarry R Beuchat
Aug 4, 2015·Environmental Science and Pollution Research International·M SeidlL Moulin
Dec 25, 2008·Yonsei Medical Journal·Jung Beom KimHiun Suk Chae
Mar 14, 2012·Journal of Food Protection·Sarah NorbergPaul D Cotter
May 9, 2012·Journal of Food Protection·Arena N RichardsonMary Alice Smith
Nov 12, 2009·Journal of Food Protection·Arena N RichardsonMary Alice Smith
Feb 5, 2005·Journal of Food Protection·O Guillaume-GentilH Joosten
Jan 7, 2005·Journal of Food Protection·A Lehner, R Stephan
May 27, 2010·Journal of Food Protection·Arena N RichardsonMary Alice Smith

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.