Comparison of fetal cartilage-derived progenitor cells isolated at different developmental stages in a rat model

Development, Growth & Differentiation
Mijin KimByoung-Hyun Min

Abstract

Fetal cartilage-derived progenitor cells (FCPCs) could be a useful cell source in cell-based therapies for cartilage disorders. However, their characteristics can vary depending on the developmental stages. The aim of this study was to compare the characteristics of rat FCPCs from the hind limb on embryonic day 14 (E14), E16 and E20 regarding proliferation, pluripotency, and differentiation. Morphologically, rat fetal cartilage tissue showed an increase in cartilaginous differentiation features (Safranin-O, type II collagen) and decrease in pluripotency marker (Sox2) in the order of E14, E16 and E20. E14 FCPCs showed significantly higher doubling time compared to E16 and E20 FCPCs. While the E14 FCPCs expressed pluripotent genes (Sox2, Oct4, Nanog), the E16 and E20 FCPCs expressed chondrogenic markers (Sox9, Col2a1, Acan). E20 FCPCs showed the highest ability to both chondrogenic and adipogenic differentiation and E14 FCPCs showed relatively better activity in osteogenic differentiation. Further analysis showed that E20 FCPCs expressed both adipogenic (C/ebpß) and osteogenic (Runx2, Sp7, Taz) transcription factors as well as chondrogenic transcription factors. Our results show an inverse relationship overall between the express...Continue Reading

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Citations

Jan 24, 2019·Stem Cells and Development·Nikola SobočanFloriana Bulić-Jakuš
Jan 4, 2019·Tissue Engineering and Regenerative Medicine·Mijin KimByoung-Hyun Min
Apr 3, 2020·Scientific Reports·Do Young ParkByung Hyune Choi
Mar 6, 2017·Tissue Engineering and Regenerative Medicine·Tung Nguyen ThanhByung Hyune Choi
Apr 3, 2021·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Dong Il ShinKyung Jun Min

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