Comparison of five agglutination tests for identification of Staphylococcus aureus.

Journal of Clinical Microbiology
M WilkersonP P Bourbeau

Abstract

Various commercially produced agglutination kits are widely used for the identification of Staphylococcus aureus. These kits detect the presence of protein A and/or clumping factor on S. aureus. The literature has shown that methicillin-resistant S. aureus (MRSA) isolates which are deficient in both clumping factor and protein A may be misidentified. Two products, Slidex and Staphaurex Plus, utilize specific anti-S. aureus antibodies, potentially giving them greater sensitivity compared to products without these antibodies. We report a prospective study designed to compare the performance characteristics of Fastaph, Slidex, Staphaurex, Staphaurex Plus, Staphyloslide, and the tube coagulase test for the identification of staphylococcal isolates. All discrepant isolates were tested with the Gen-Probe AccuProbe S. aureus test and were identified to the species level with conventional reference biochemicals. A total of 1,193 isolates were tested, including 33 MRSA and 423 methicillin-sensitive S. aureus isolates. The sensitivities and specificities of the tests, respectively, were as follows: Fastaph, 99.1 and 98.9%; Slidex, 99.6 and 96.4%; Staphaurex, 98.9 and 99.9%; Staphaurex Plus, 99.6 and 93.9%; Staphyloslide, 99.1 and 98.9%; ...Continue Reading

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Citations

Dec 2, 2010·The Journal of Dairy Research·Omer AkinedenEwald Usleber
Nov 25, 2017·BMC Complementary and Alternative Medicine·Najma ArshadHumera Afrasiab
Sep 29, 2006·Journal of Clinical Microbiology·Agnieszka Luczak-KadlubowskaWaleria Hryniewicz
Mar 21, 1998·Journal of Clinical Microbiology·F MartineauM G Bergeron
Feb 13, 1999·Journal of Clinical Microbiology·T A WichelhausV Brade
Jan 5, 2005·Diagnostic Microbiology and Infectious Disease·Anna-Maria CostaSilvano Palladino
Mar 22, 2005·Archives of Medical Research·Roberto Rivera-SánchezMyriam Arriaga-Alba

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