Comparison of five derivatizing agents for the determination of amphetamine-type stimulants in human urine by extractive acylation and gas chromatography-mass spectrometry

Journal of Analytical Toxicology
Adrienn DobosGábor Pál Somogyi

Abstract

Five acylation reagents have been compared for use as derivatizing agents for the analysis of amphetamine-type stimulants (ATS) in urine by gas chromatography-mass spectrometry (GC-MS). The evaluated reagents were heptafluorobutyric anhydride, pentafluoropropionic anhydride, trifluoroacetic anhydride, acetic anhydride (AA) and N-methyl-bis(trifluoroacetamide). The ATS included amphetamine, methamphetamine (MA), 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA) and 3,4-methylenedioxyethylamphetamine (MDEA). A mixture of the ATS was added to urine (1 mL) followed by KOH solution and saturated NaHCO(3) solution. The sample was then extracted with dichloromethane and the derivatizing agent and 2 µL were injected into the GC-MS instrument. The derivatizing agents were compared with reference to the signal-to-noise (S/N) ratios, peak area values, relative standard deviations (RSDs), linearities, limits of detection (LODs) and selectivities. The acetic anhydride proved to be the best according to the S/N ratio and peak area results for amphetamine, MA, MDMA and MDEA. The best RSD values of peak areas and of S/N ratios at 3 µg/mL were also given by AA in cases of MDA, MDMA and MDEA. At 20 µg/mL, the lowest R...Continue Reading

References

Sep 11, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Aino KankaanpääTimo Seppälä
Jul 15, 2006·Forensic Science International : Synergy·Elod HidvégiGábor Somogyi
Mar 18, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Akihiro MikiHitoshi Tsuchihashi
Dec 17, 2008·Forensic Science International : Synergy·Adriaan A S Marais, Johannes B Laurens
Jan 5, 2011·Journal of Pharmaceutical and Biomedical Analysis·Hiroyuki Kataoka, Keita Saito

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Citations

Jun 20, 2017·British Journal of Biomedical Science·N W Brown
Aug 10, 2017·The Analyst·Haoxuan ZhuJ Scott McIndoe
Dec 15, 2012·Rapid Communications in Mass Spectrometry : RCM·Jon K DalgleishR Graham Cooks

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