PMID: 8459033Feb 1, 1993Paper

Comparison of flow and static image cytometry in the determination of ploidy

Journal of Clinical Pathology
D LaniganM Leader

Abstract

To compare the efficiency of flow cytometry and computed image cytometry; and to see if a reliable set of guidelines regarding interpretation of histograms could be drawn up. The two methods were applied to a series of 111 formalin fixed renal cell carcinomas. Data generated by both methods were compared. The methods agreed in 85 cases. Hypodiploidy was detected by computed image cytometry in seven cases in which flow cytometry had shown only an aneuploid peak. Aneuploidy in seven in which the corresponding flow cytometry histogram was diploid. There was an overlap in the second peak proportions on flow cytometry histograms between those classed as diploid or tetraploid by computed image cytometry. In six cases the flow cytometry histograms had unacceptably high coefficients of variation and in all of these cases computed image cytometry demonstrated aneuploidy. Computed image cytometry is particularly useful for clarifying difficult areas in flow histograms--specifically, high coefficients of variation, high G2M phase, as well as possible near diploid aneuploidy and hypodiploidy.

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Citations

Dec 1, 1994·Genes, Chromosomes & Cancer·M J DonovanD E Schofield
Apr 14, 2005·Virchows Archiv : an International Journal of Pathology·Zsombor MeleghZoltán Szentirmay
Jun 1, 1994·Cancer Genetics and Cytogenetics·M ZandeckiA Cosson
Jun 27, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Linyan XieXuantao Su
Nov 25, 1997·Japanese Journal of Cancer Research : Gann·N FutakawaY Esaki
Nov 6, 2009·Risk Analysis : an Official Publication of the Society for Risk Analysis·Denita WilliamsMary Alice Smith
Aug 31, 2012·Journal of the Royal Society, Interface·V Medici, S N Fry
Jul 6, 2019·Frontiers in Bioengineering and Biotechnology·Abhishek VembadiMohammad A Qasaimeh
Oct 22, 2016·International Journal of Colorectal Disease·Rüdiger MeyerJens K Habermann
May 4, 2020·World Journal of Urology·Franziska BüscheckMichael Rink

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