PMID: 8946667Dec 1, 1996Paper

Comparison of fluorescence polarization immunoassay with liquid chromatography for quantification of procainamide and N-acetylprocainamide in urine

Therapeutic Drug Monitoring
J E TisdaleC K Svensson

Abstract

The objective of this study was to compare the precision and accuracy of fluorescence polarization immunoassay (FPIA) with high-performance liquid chromatography (HPLC) for measurement of procainamide (PA) and N-acetylprocainamide (NAPA) concentrations in urine. To determine the correlation between FPIA and HPLC, urine PA and NAPA concentrations were assayed using both techniques in samples obtained from study patients receiving PA and in spiked samples. In samples from patients, FPIA-determined PA and NAPA concentrations were 19 +/- 9% lower and 28 +/- 31% higher, respectively, than those determined by HPLC. The slope of the FPIA-HPLC regression lines for PA and NAPA differed significantly from that of the line of unity (the slope that would result if FPIA and HPLC yielded identical concentrations). In spiked samples, FPIA-determined PA and NAPA concentrations were 15 +/- 2% and 11 +/- 2% lower than HPLC-determined concentrations, respectively, and the slopes of the FPIA-HPLC regression lines differed significantly from the line of unity. Therefore, FPIA cannot be recommended as a urine assay method when quantitative assessment of urine PA or NAPA excretion is needed for pharmacokinetic studies.

References

Nov 1, 1977·Clinical Pharmacology and Therapeutics·P du Souich, S Erill
Jan 1, 1978·Clinical Pharmacology and Therapeutics·J J Lima, W J Jusko
Jan 1, 1991·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·W G Wood
Aug 1, 1988·Journal of Pharmaceutical Sciences·L L LiuC K Svensson
Jan 1, 1984·Therapeutic Drug Monitoring·A M Al-FaresY M el-Sayed
Jan 1, 1996·Journal of Clinical Pharmacology·J E TisdaleB J Zarowitz

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Citations

Oct 31, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mugunthu R DhananjeyanPaul W Erhardt

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