Comparison of full-length sequences of interferon-sensitive and resistant hepatitis C virus 1b. Sensitivity to interferon is conferred by amino acid substitutions in the NS5A region

The Journal of Clinical Investigation
N EnomotoC Sato

Abstract

We have previously demonstrated that sensitivity to interferon is different among hepatitis C virus (HCV) quasispecies simultaneously detected in same individuals and that interferon-resistant HCV quasispecies are selected during the treatment. To determine the genetic basis of their resistance to interferon, HCV genotype-1b was obtained from serum of three patients before and during interferon therapy, and their full-length nucleotide and deduced amino acid sequences were determined. Comparison of the pairs of interferon-resistant and interferon-sensitive HCV isolates in respective individuals demonstrated clusters of amino acid differences in the COOH-terminal half of the NS5A region (codon 2154-2383), which contained a common unique amino acid difference at codon 2218. Additional sequence data of the COOH-terminal half of the NS5A region obtained from six interferon-resistant and nine interferon-sensitive HCV confirmed the exclusive existence of missense mutations in a 40 amino acid stretch of the NS5A region around codon 2218 (from codon 2209 to 2248) in interferon-sensitive HCV. On the other hand, this region of interferon-resistant HCV was identical to that of prototype HCV genotype-1b (HCV-J, HCV-JTa, or HC-J4). We desig...Continue Reading

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Citations

Jul 1, 1996·Journal of Medical Virology·R P González-PeraltaJ Y Lau
Oct 2, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T FukumaC Sato
Nov 26, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·L FrangeulJ M Huraux
Aug 26, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K R ReddyT Joh
Sep 25, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T MurakamiC Sato
Feb 22, 2002·Journal of Medical Virology·Byung-Il YehJong-Whan Choi
Feb 11, 1998·Liver Transplantation and Surgery : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·R LeonE R Schiff
Jan 1, 1997·Springer Seminars in Immunopathology·P FarciR H Purcell
Jan 1, 1997·Springer Seminars in Immunopathology·K M ChangF V Chisari
Jan 1, 1996·Archives of Virology·I SakumaC Sato
Apr 1, 1996·Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme·N Kato, K Shimotohno
Jan 1, 1997·International Journal of Clinical & Laboratory Research·H E Blum
Jun 21, 2005·Journal of Hepatology·Ralf Bartenschlager
Jul 2, 2003·Antiviral Research·Jean Michel Pawlotsky
Sep 3, 2003·Virus Research·Adrish SenRatna B Ray
Jan 24, 2002·Journal of Hepatology·Maria Stella De MitriMauro Bernardi
Jan 6, 2000·Journal of Hepatology·R De Francesco
Jan 6, 2000·Journal of Hepatology·H C ThomasS D Taylor-Robinson
Feb 7, 2012·Enfermedades infecciosas y microbiología clínica·Verónica Saludes MontoroElisa Martró Català
Sep 28, 2002·FEMS Microbiology Letters·M P H Stumpf, O G Pybus

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