PMID: 59610Jun 7, 1976Paper

Comparison of fungal glucose oxidases. Chemical, physicochemical and immunological studies

Biochimica Et Biophysica Acta
S Hayashi, S Nakamura

Abstract

Glucose oxidases (beta-D-glucose:oxygen 1-oxidoreductase, EC 1.1.3.4) from two fungal genera (Aspergillus and Penicillium) were studied chemically, physicochemically and immunologically to elucidate the similarities and dissimilarities between these enzymes. Investigation of circular dichroism spectra revealed that these enzymes proteins possess essentially identical conformations. However, differences found in thermal inactivation parameters, catalytic parameters and quantitative immunological reactivities indicate that these enzymes must have some minor but distinct variations in their structures. Interestingly, it was observed that the Penicillium enzyme cross-reacted with the antiserum against the Aspergillus enzyme with an association constant of two orders of magnitude lower than that of the Aspergillus enzyme, and that the precipitin one of the Penicillium enzyme fused together with that of the Aspergillus enzyme in the immunodouble diffusion test. These results lead to the conclusion that these enzymes are closely related but not completely identical, and suggest that they might have evolved from a common ancestral precursor.

Citations

Sep 3, 2013·Applied Biochemistry and Biotechnology·T V SemashkoArunas Ramanavicius
Jan 15, 2008·Journal of Clinical Biochemistry and Nutrition·Muhammad Anjum ZiaAzeem I Khan
Nov 25, 2011·Enzyme and Microbial Technology·Michael S CavesRobert B Freedman
Jul 1, 2018·Biotechnology and Bioengineering·Erik Arango GutierrezUlrich Schwaneberg
Jul 25, 2013·Biofabrication·Jonathan DudzikGerald F Audette

Related Concepts

Antigenic Specificity
Aspergillus
Aspergillus niger
Sugars
Immunodiffusion Measurement
Glucose Oxidase
Immune Sera
Penicillium
Protein Conformation
Thermodynamics

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