Comparison of GC-MS and GC×GC-MS in the analysis of human serum samples for biomarker discovery

Journal of Proteome Research
Jason H WinnikeXiang Zhang

Abstract

We compared the performance of gas chromatography time-of-flight mass spectrometry (GC-MS) and comprehensive two-dimensional gas chromatography mass spectrometry (GC×GC-MS) for metabolite biomarker discovery. Metabolite extracts from 109 human serum samples were analyzed on both platforms with a pooled serum sample analyzed after every 9 biological samples for the purpose of quality control (QC). The experimental data derived from the pooled QC samples showed that the GC×GC-MS platform detected about three times as many peaks as the GC-MS platform at a signal-to-noise ratio SNR ≥ 50, and three times the number of metabolites were identified by mass spectrum matching with a spectral similarity score Rsim ≥ 600. Twenty-three metabolites had statistically significant abundance changes between the patient samples and the control samples in the GC-MS data set while 34 metabolites in the GC×GC-MS data set showed statistically significant differences. Among these two groups of metabolite biomarkers, nine metabolites were detected in both the GC-MS and GC×GC-MS data sets with the same direction and similar magnitude of abundance changes between the control and patient sample groups. Manual verification indicated that the difference in ...Continue Reading

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Citations

Jul 29, 2015·Journal of Proteome Research·Xiaoli WeiXiang Zhang
May 2, 2016·Journal of Chromatography. a·Imhoi KooXiang Zhang
Jun 7, 2018·Rapid Communications in Mass Spectrometry : RCM·Biswapriya B MisraMichael Olivier
Jan 30, 2020·Biomedical Chromatography : BMC·Zhendong ChenDafang Zhong
Aug 14, 2020·Metabolomics : Official Journal of the Metabolomic Society·Nicolas Di GiovanniJean-François Focant
Sep 12, 2017·Metabolomics : Official Journal of the Metabolomic Society·Rachel SpicerChristoph Steinbeck
Mar 5, 2019·Metabolomics : Official Journal of the Metabolomic Society·Biswapriya B MisraMichael Olivier
Mar 17, 2019·Multiple Sclerosis and Related Disorders·S L AndersenS G Gregory

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