Comparison of gene expression in old versus young rat hippocampus by cDNA array

Neuroreport
Kyung Sook ChoByung Ju Lee

Abstract

We sought to identify genes affected by the aging process in the rat hippocampus using cDNA expression array analysis. RNA samples were extracted from the hippocampus of 2-month-old and 20-month-old rats and reverse-transcribed in the presence of [32P]dCTP. Membrane sets of Rat Atlas array 2 (Clontech) were hybridized with cDNA probe sets. Among a total of 1176 cDNAs, 23 showed significant (more than 2-fold) changes between groups. Eight genes were increased in the old group, while the remaining fifteen genes were decreased. Reverse transcription-polymerase chain reaction (RT-PCR) was used to validate the relative expression pattern obtained by the cDNA array. The results were consistent for 20 of the 23 genes tested. Most interesting findings were the decrease in expression of proteins for energy metabolism, proteins involved in secretion, and ribosomal proteins. The possible physiological significance of these changes are discussed.

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Citations

May 14, 2005·Neurobiology of Aging·Matthew G FrankSteven F Maier
Nov 6, 2002·Journal of Neuroimmunology·Akira TeraoYvonne R Freund
May 10, 2005·International Journal of Cancer. Journal International Du Cancer·Seung Koo LeeJeong Woo Park
Sep 24, 2004·Journal of Neurochemistry·Yoshinori MasuoEtsuo Niki
Jul 6, 2007·The European Journal of Neuroscience·Yoshinori MasuoEtsuo Niki
Apr 20, 2004·European Journal of Pharmacology·Siegfried Hoyer
Jan 3, 2006·Neurobiology of Aging·Myriel NyffelerIrene Knuesel

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