Comparison of gene expression profile of the spinal cord of sprouting-capable neonatal and sprouting-incapable adult mice

BMC Genomics
Hiroshi Tsujioka, Toshihide Yamashita

Abstract

The regenerative ability of severed axons in the central nervous system is limited in mammals. However, after central nervous system injury, neural function is partially recovered by the formation of a compensatory neural circuit. In a mouse pyramidotomy model, axonal sprouting of the intact side of the corticospinal tract is observed in the spinal cord, and the axons make new synapses with the denervated side of propriospinal neurons. Moreover, this sprouting ability is enhanced in neonatal mice compared to that in adult mice. Myelin-associated molecules in the spinal cord or intrinsic factors in corticospinal neurons have been investigated in previous studies, but the factors that determine elevated sprouting ability in neonatal mice are not fully understood. Further, in the early phase after pyramidotomy, glial responses are observed in the spinal cord. To elucidate the basal difference in the spinal cord, we compared gene expression profiles of entire C4-7 cervical cord tissues between neonatal (injured at postnatal day 7) and adult (injured at 8 weeks of age) mice by RNA-sequencing. We also tried to identify discordant gene expression changes that might inhibit axonal sprouting in adult mice at the early phase (3 days) aft...Continue Reading

References

Dec 1, 1995·Neurodegeneration : a Journal for Neurodegenerative Disorders, Neuroprotection, and Neuroregeneration·S K LeongD P Fan
May 26, 1999·Progress in Neurobiology·G Stoll, S Jander
Jun 22, 2002·Trends in Biochemical Sciences·Naohiro Inohara, Gabriel Nuñez
Mar 18, 2003·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Hisayuki NomiyamaKatsuyuki Hashimoto
Jul 30, 2005·Experimental Neurology·Michelle L StarkeyElizabeth J Bradbury
Aug 10, 2005·Journal of Neuroimmunology·Motoko KannoJunya Tanaka
Jan 15, 2008·Biological Psychiatry·Ling Shao, Marquis P Vawter
Jul 25, 2008·PLoS Biology·Konstantinos MeletisJonas Frisén
Sep 19, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Irin C MaierMartin E Schwab
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
May 29, 2009·BMC Bioinformatics·Weijun LuoPeter J Woolf
Jun 26, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Laila ZaiLarry I Benowitz
Jun 8, 2010·BMC Neuroscience·Justin R SiebertDennis J Stelzner
Jul 20, 2010·Methods : a Companion to Methods in Enzymology·Akiya WatakabeTetsuo Yamamori
Oct 1, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Shusaku OmotoToshihide Yamashita
Oct 22, 2010·The Journal of Comparative Neurology·Valerie C SiembabFrancisco J Alvarez
Nov 19, 2010·Blood·Felix EllettGraham J Lieschke
Feb 2, 2011·Immunobiology·Govindarajan ThangaveluColin C Anderson
Jun 30, 2011·Developmental Neurobiology·Jonathan P MyersTimothy M Gomez
Jan 14, 2012·The Journal of Biological Chemistry·Maria Veiga-da-CunhaEmile Van Schaftingen
Jun 1, 2012·Developmental Neurobiology·Adam J SvahnThomas S Becker
Mar 28, 2013·Annals of the New York Academy of Sciences·Francisco J AlvarezValerie C Siembab
Jun 12, 2013·Infection and Immunity·K A FieldsE R Podack
Aug 31, 2013·BioMed Research International·Kanako ShibataSumio Tanase
Jan 30, 2014·Annual Review of Immunology·Debasis NayakDorian B McGavern
Jun 4, 2014·Cold Spring Harbor Perspectives in Biology·E Richard Stanley, Violeta Chitu
Jul 16, 2014·Experimental Neurology·Kristina A KigerlRobert W Keane
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber
Oct 3, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Linard FilliMartin E Schwab
Nov 14, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Do-Hun LeeKevin K Park
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Mar 10, 2015·Nature Methods·Daehwan KimSteven L Salzberg
Mar 10, 2016·Experimental Neurology·Jenna M Gaesser, Sharyl L Fyffe-Maricich
Jun 30, 2016·International Immunology·Richard Thomas JenningsSachiko Akashi-Takamura
Jul 17, 2016·Ageing Research Reviews·Santiago Solé-DomènechFrederick R Maxfield

❮ Previous
Next ❯

Citations

Dec 4, 2020·International Immunology·Hiroshi Tsujioka, Toshihide Yamashita
Apr 13, 2021·Frontiers in Neuroscience·Lvwan XuLin-Lin Wang
Jun 3, 2021·Cell and Tissue Research·Amanda Phuong TranJerry Silver

❮ Previous
Next ❯

Methods Mentioned

BETA
RNA-seq
transgenic
PCR
acetylation

Software Mentioned

gage
DESeq2
HISAT2
DAVID
ggplot2
ggbeeswarm
HTSeq
R

Related Concepts

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.