PMID: 8609196Nov 1, 1995Paper

Comparison of genotyping and serotyping methods for the identification of hepatitis C virus types

Journal of Virological Methods
S TisminetzkyA Alberti

Abstract

The usefulness of identification of hepatitis C virus (HCV) genotype has recently been investigated for the clinical management of patients infected by HCV. In the present study, the HCV genotype infecting 127 patients was determined by two different methods: HCV genotyping using a dot-blot assay with type-specific probes derived from the 5'-UTR of HCV genome and HCV serotyping using an ELISA system in which type-specific antibodies against the NS4 region were detected. Overall, a good correlation of the two methods was observed, the main discrepancy being 4 patients with sequence-confirmed HCV-2 (2 cases) and HCV-3 (2 cases) genotypes recognized as HCV-1 by serotyping. Mixed infections were not detected by either method. In 19 PCR negative sera, in which the HCV genotype could not be evaluated, no particular serotype profile was observed. In conclusion, the molecular and serological techniques are almost equivalent in determining the viral type, although in individual cases, especially in PCR negative patients, the clinical meaning of the serotyping result remains to be determined.

References

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Citations

Feb 1, 1996·Journal of Medical Virology·P PontissoA Alberti
Apr 1, 1996·Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme·N Kato, K Shimotohno
May 18, 2000·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·G StantaC Tiribelli
Jul 1, 1997·Journal of Gastroenterology and Hepatology·D B Smith, P Simmonds
Dec 1, 1996·Journal of Clinical Pathology·L M WebberW O Grabow
Apr 15, 2006·International Journal of Medical Sciences·Zhihua Liu, Jinlin Hou
Aug 5, 1998·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·G LodiC Scully
Jul 28, 2011·Virology Journal·Mehwish RiazFiroz Kabir
May 5, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·F CalabreseM Valente
Mar 3, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·L FanningF Shanahan
Mar 26, 2003·The American Journal of Gastroenterology·Maria GuidoMassimo Rugge
Nov 18, 2018·Clinical Journal of Gastroenterology·Masako OkadaNorifumi Kawada
Sep 8, 1998·Clinical and Diagnostic Laboratory Immunology·S SongsivilaiT Dharakul

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