Comparison of gyrA mutations, cyclohexane resistance, and the presence of class I integrons in Salmonella enterica from farm animals in England and Wales.

Journal of Clinical Microbiology
Ernesto LiebanaRobert H Davies

Abstract

This study is focused on real-time detection of gyrA mutations and of the presence of class I integrons in a panel of 100 veterinary isolates of Salmonella enterica from farm animals. The isolates were selected on the basis of resistance to nalidixic acid, representing a variety of the most prevalent serotypes in England and Wales. In addition, organic solvent (cyclohexane) resistance in these isolates was investigated in an attempt to elucidate the presence of efflux pump mechanisms. The most prevalent mutation among the isolates studied was Asp87-Asn (n = 42), followed by Ser83-Phe (n = 38), Ser83-Tyr (n = 12), Asp87-Tyr (n = 4), and Asp87-Gly (n = 3). Two distinct subpopulations were identified, separated at the 1-mg/liter breakpoint for ciprofloxacin: 86% of isolates with mutations in codon 83 showed MICs of >or=1 mg/liter, while 89.8% of isolates with mutations in codon 87 presented MICs of <or=0.5 mg/liter. Cyclohexane resistance was more prevalent among Ser83 mutants than among Asp87 mutants (34.7 and 4%, respectively), and in 79% of isolates that presented both gyrA mutations and cyclohexane resistance, the level of ciprofloxacin resistance was >or=2.0 mg/liter. Thirty-four isolates contained class I integrons, with 71%...Continue Reading

References

Oct 1, 1987·Epidemiology and Infection·L R WardB Rowe
Jun 1, 1980·Journal of Clinical Pathology·C R Shipp, B Rowe
Jul 1, 1995·Antimicrobial Agents and Chemotherapy·F ReynaL Y Fuchs
Jan 1, 1993·Drugs·E Cambau, L Gutmann
Apr 1, 1996·Antimicrobial Agents and Chemotherapy·D J GriggsL J Piddock
Apr 1, 1997·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·B McCarron, W C Love
Oct 1, 1995·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·P HeisigJ P Faber
Jul 1, 1997·Emerging Infectious Diseases·H HerikstadF J Angulo
Mar 21, 1998·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·F J VasalloE Bouza
Jul 1, 1998·Infection·A VinceM Radovani
Jan 5, 1999·Applied and Environmental Microbiology·H AsakoR Aono
Nov 5, 1999·The New England Journal of Medicine·K MølbakH C Wegener
Jun 27, 2000·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·C WiuffF M Aarestrup
Mar 7, 2001·Applied and Environmental Microbiology·L P Randall, M J Woodward

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Citations

Aug 20, 2011·Tropical Animal Health and Production·Shweta SinghHimanshu Singh
Mar 26, 2003·Antimicrobial Agents and Chemotherapy·Marie-Cécile PloyThierry Lambert
May 23, 2003·Antimicrobial Agents and Chemotherapy·Beatriz GuerraReiner Helmuth
May 20, 2008·Journal of Bacteriology·Julieta BarchiesiEleonora García Véscovi
Oct 9, 2004·Journal of Clinical Microbiology·Ernesto LiebanaJaime Martinez-Urtaza
Apr 14, 2004·Emerging Infectious Diseases·Po-Ren HsuehKwen-Tay Luh
Sep 2, 2004·Emerging Infectious Diseases·Amy Gassama-SowMarie-Cécile Ploy
Jun 5, 2004·Journal of Microbiological Methods·Hidetake EsakiToshio Takahashi
Aug 29, 2006·Journal of Microbiological Methods·Katie L HopkinsE John Threlfall
Apr 26, 2005·International Journal of Antimicrobial Agents·Katie L HopkinsE John Threlfall
Apr 3, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A C Fluit, F-J Schmitz
Apr 17, 2019·Journal of Medical Microbiology·Megha KaushikPooja Gulati
Jul 23, 2004·The Journal of Antimicrobial Chemotherapy·L P RandallM J Woodward
Jul 10, 2004·The Journal of Antimicrobial Chemotherapy·L P RandallM J Woodward
Mar 18, 2006·Journal of Food Protection·S ZhaoD G White

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