Comparison of healing process in open osteotomy model and open fracture model: Delayed healing of osteotomies after intramedullary screw fixation

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
Moritz KleinTina Histing

Abstract

Murine osteotomy and fracture models have become the standard to study molecular mechanisms of bone healing. Because there is little information whether the healing of osteotomies differs from that of fractures, we herein studied in mice the healing of femur osteotomies compared to femur fractures. Twenty CD-1 mice underwent a standardized open femur osteotomy. Another 20 mice received a standardized open femur fracture. Stabilization was performed by an intramedullary screw. Bone healing was studied by micro-CT, biomechanical, histomorphometric and protein expression analyses. Osteotomies revealed a significantly lower biomechanical stiffness compared to fractures. Micro-CT showed a reduced bone/tissue volume within the callus of the osteotomies. Histomorphometric analyses demonstrated also a significantly lower amount of osseous tissue in the callus of osteotomies (26% and 88% after 2 and 5 weeks) compared to fractures (50% and 100%). This was associated with a delayed remodeling. Western blot analyses demonstrated comparable BMP-2 and BMP-4 expression, but higher levels of collagen-2, CYR61 and VEGF after osteotomy. Therefore, we conclude that open femur osteotomies in mice show a markedly delayed healing when stabilized les...Continue Reading

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Citations

Feb 19, 2016·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·T HistingM D Menger
May 24, 2017·The Journal of International Medical Research·Wei-Long LiQing-Jiang Pang
Nov 22, 2017·BMC Musculoskeletal Disorders·Stefanie Fitschen-OesternClaudia Neunaber
Aug 6, 2020·Nanomaterials·Shuai YueTao Hou
Feb 10, 2020·Archives of Orthopaedic and Trauma Surgery·Jens EverdingMichael Raschke
Aug 25, 2020·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Christopher D KegelmanJoel D Boerckel
Jul 3, 2021·Biomedicines·Moritz PfeiffenbergerTimo Gaber

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