Comparison of Helicobacter pylori and Escherichia coli in induction of TNF-alpha mRNA from human peripheral blood mononuclear cells

Indian Journal of Medical Microbiology
E AhmadzadehF Navab Akbar

Abstract

To investigate the difference between the abilities of Helicobacter pylori and Escherichia coli to induce expression of TNF-alpha in human peripheral blood mononuclear cells (PBMC). H pylori was isolated from gastric biopsy specimens. The mononuclear cells were isolated from human blood, cultured, and treated with either intact or sonicated E coli or H pylori, and mRNA expression for TNF-alpha was detected using semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). TNF-alpha mRNA expression levels were significantly higher in PBMCs stimulated with E coli compared to those stimulated with H pylori at the same number and identical conditions (P < .001). The results also suggest that sonicated bacteria were significantly (P < .001) less stimulatory for PBMCs than intact bacteria for both E coli and H pylori. The ability of different H pylori strains isolated from biopsy samples to stimulate TNF-alpha from PBMCs was significantly lower than that of E coli. Sonicated bacteria, as compared to intact bacteria, was a very poor inducer of TNF-alpha mRNA expression, suggesting that the conformation of lipopolysaccharides (LPS) on the outer leaflet of the outer membrane is not totally conserved in sonicated bacteria.

References

Apr 1, 1992·Infection and Immunity·A MuotialaA P Moran
Aug 24, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·M GöõzA J Smolka
Oct 9, 2001·Scandinavian Journal of Gastroenterology·C LindholmA M Svennerholm
Oct 16, 2001·Journal of Medical Microbiology·N MatsuyamaM Nakano
Jul 11, 2002·Microbes and Infection·Martine CaroffNicole Haeffner-Cavaillon
May 3, 2003·FEMS Immunology and Medical Microbiology·Tomohiko OgawaToshihide Tamura
Jun 1, 2005·Carbohydrate Research·Stacey BrittonMario A Monteiro
Jun 24, 2005·Infection and Immunity·Katharina KranzerWulf Schneider-Brachert
Nov 8, 2005·World Journal of Gastroenterology : WJG·Marjan MohammadiSadegh Massarrat
Sep 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Xiaoren TangSalomon Amar

❮ Previous
Next ❯

Citations

Jun 14, 2013·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Karolina RudnickaMagdalena Chmiela
Jun 19, 2013·Microbiology and Immunology·Hamid Reza RahimiSimin Kiany
Nov 22, 2020·Journal of Clinical Medicine·Nozomi ItoNariyoshi Shinomiya
Jul 3, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Kai ChenYing Zhu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.