Comparison of herpes simplex virus reactivation in ganglia in vivo and in explants demonstrates quantitative and qualitative differences

Journal of Virology
N M Sawtell, R L Thompson

Abstract

The in vivo ganglionic environment directs the latent herpes simplex virus transcriptional program. Since stress-driven perturbations in sensory neurons are thought to play a critical role in the transition from latency to reactivation, a primary concern in the selection of a valid model of the molecular interactions leading to reactivation is the faithful recapitulation of these environments. In this study reactivation of latently infected ganglia excised and cultured in vitro (explanted) is compared to reactivation occurring in latently infected ganglia in vivo following hyperthermic stress. Three notable points emerged. (i). Neurons in explanted ganglia exhibited marked morphological changes within 2 to 3 h postexplant. DNA fragmentation in neuronal nuclei was detected at 3 h, and atypical expression of cell cycle- and stress-regulated proteins such as geminin, cdk2, cdk4, and cytochrome c became apparent at 2 to 48 h. These changes were associated with axotomy and explant and not with the initiation or progression of reactivation and were not observed in ganglia following in vivo hyperthermic stress. (ii). Despite these differences, during the first 22 h primary reactivation events were restricted to a very small number of ...Continue Reading

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Citations

Apr 14, 2010·Cell Research·Gautam R BedadalaShao-Chung V Hsia
Mar 28, 2009·PLoS Pathogens·Richard L ThompsonNancy M Sawtell
Feb 18, 2010·Future Virology·D Curanovic, Lw Enquist
Sep 22, 2011·Diseases of Aquatic Organisms·Ana B Arnizaut, Larry A Hanson
Jul 19, 2013·Viruses·Zachary WatsonDavid Bloom
Aug 26, 2014·PloS One·Simone C WuestBibiana Bielekova
Apr 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Leigh ZerboniAnn M Arvin
Mar 22, 2015·The Journal of General Virology·Peter G E KennedyRandall J Cohrs
Sep 12, 2012·Trends in Microbiology·Angus C Wilson, Ian Mohr
Jan 4, 2018·MBio·Luis M Schang
Mar 7, 2020·PLoS Pathogens·Jessica R DollNancy M Sawtell
Mar 22, 2019·Journal of Virology·Julianna R PieknikPhilip R Krause
Dec 2, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gregory M FrankRobert L Hendricks
Aug 20, 2020·Nature Communications·Martine AubertKeith R Jerome
Jul 4, 2018·Future Virology·Orkide O KoyuncuLynn W Enquist

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