Comparison of high-performance liquid chromatography with fluorescence polarization immunoassay for the analysis of vancomycin in patients with chronic renal failure

Journal of Chromatography. B, Biomedical Applications
T A NajjarA Tekle

Abstract

Eighty-two plasma samples from patients with chronic renal failure undergoing vancomycin treatment and hemodialysis (HD) were analyzed with fluorescence polarization immunoassay (FPIA) and high-performance liquid chromatography (HPLC). Vancomycin was infused once and the samples were collected during three subsequent HD sessions at 2 h, 3 days and 5 days post-infusion. The HPLC method, modified from an earlier assay, was simple. There was a wide variation in the estimated concentration between the two assay methods. The results obtained by HPLC were 69% lower than those obtained by FPIA. This difference in vancomycin concentration was independent of the sampling time after vancomycin infusion. HPLC analysis commenced approximately 1.5 year after that of FPIA. To study the effect of in vitro degradation, the vancomycin concentration in ten of the samples was redetermined with FPIA during HPLC analysis. The concentrations of those samples decreased to 78-98% (average 92%) of the original concentration. Because FPIA appears to lack specificity, there is a need of other methods such as HPLC for vancomycin measurements, particularly in samples from patients with end-stage renal failure.

Citations

Oct 6, 2000·Clinica Chimica Acta; International Journal of Clinical Chemistry·I FurutaY Morishima
May 29, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Paul MetzD N Gilbert
Apr 24, 2002·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Masayo TanakaShuichi Hirono
Jan 12, 2000·The Annals of Pharmacotherapy·P F Smith, G D Morse
Mar 30, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jiajing WangGuorong Fan
Oct 27, 2017·Journal of Chromatographic Science·Tácio de Mendonça LimaRita de Cássia Elias Estrela
Feb 18, 1998·Clinical Pharmacology and Therapeutics·F Schaedeli, D E Uehlinger

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