Comparison of HLA-DR1-restricted T cell response induced in HLA-DR1 transgenic mice deficient for murine MHC class II and HLA-DR1 transgenic mice expressing endogenous murine MHC class II molecules

International Immunology
Anthony PajotYu-Chun Lone

Abstract

Transgenic mice expressing human HLA class II molecules provide a useful model for identifying HLA-restricted CD4+ epitopes. However, the influence of endogenous murine H-2-restricted T cell responses on HLA-restricted responses is not known. In the present study, we show that HLA-DR1 transgenic mice deficient for H-2 class II expression (HLA-DR1+/+/IAbeta0/0) exhibit an equivalent expression level of the transgene HLA-DR1 and a similar diversity in the TCR repertoire, but a slightly different number of CD4+ peripheral T cells, when compared to HLA-DR1 transgenic mice in which H-2 class II molecules were retained (HLA-DR1+/+/IAbeta+/+). More importantly, a strong antigen-specific HLA-DR1-restricted response was observed in nearly all HLA-DR1+/+/IAbeta0/0 mice immunized with HBV envelope protein (HBs) or capsid protein (HBc), whereas weak HBs- or HBc-specific HLA-DR1-restricted responses were detected in half of the immunized HLA-DR1+/+/IAbeta+/+ mice. Conversely, strong HBs- or HBc-specific H-2-restricted T cell responses were detected in HLA-DR1+/+/IAbeta+/+ mice but not in HLA-DR1+/+/IAbeta0/0 mice. Our results indicate that the coexpression of endogenous H-2 class II molecules reduces the intensity of HLA-DR1-restricted anti...Continue Reading

Citations

Sep 16, 2004·The Journal of Chemical Physics·Oliver Y H TaiAlex K Y Jen
Aug 13, 2005·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Umesh S DeshmukhShu Man Fu
Oct 19, 2006·Archives of Medical Research·Mario García-CarrascoRicard Cervera
Aug 3, 2007·European Journal of Immunology·Anthony PajotYu-Chun Lone
Aug 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Olivier AdoteviEric Tartour
Aug 6, 2021·Expert Review of Vaccines·Irina Isakova-SivakLarisa Rudenko

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