Sep 17, 2016

Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions

Experimental Biology and Medicine
Otilija KetaAleksandra M Ristić Fira

Abstract

The aim of this study was to investigate effects of irradiations with the therapeutic proton and carbon ion beams in two non-small cell lung cancers, CRL5876 adenocarcinoma and HTB177 large cell lung carcinoma. The DNA damage response dynamics, cell cycle regulation, and cell death pathway activation were followed. Viability of both cell lines was lower after carbon ions compared to the therapeutic proton irradiations. HTB177 cells showed higher recovery than CRL5876 cells seven days following the treatments, but the survival rates of both cell lines were lower after exposure to carbon ions with respect to therapeutic protons. When analyzing cell cycle distribution of both CRL5876 and HTB177 cells, it was noticed that therapeutic protons predominantly induced G1 arrest, while the cells after carbon ions were arrested in G2/M phase. The results illustrated that differences in the levels of phosphorylated H2AX, a double-strand break marker, exist after therapeutic proton and carbon ion irradiations. We also observed dose- and time-dependent increase in the p53 and p21 levels after applied irradiations. Carbon ions caused larger increase in the quantity of p53 and p21 compared to therapeutic protons. These results suggested that v...Continue Reading

  • References50
  • Citations3

References

  • References50
  • Citations3

Citations

Mentioned in this Paper

TP53 gene
Biological Markers
Study
Biochemical Pathway
Adenocarcinoma of Lung (Disorder)
H2AFX gene
Proton Therapy
H2AFX wt Allele
Oncoprotein p21
Vitreous Carbon

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