Comparison of humoral and T-cell-mediated immune responses to a single dose of Bovela(®) live double deleted BVDV vaccine or to a field BVDV strain

Veterinary Immunology and Immunopathology
Ratree PlattJames A Roth

Abstract

The objective of this study was to determine and compare the humoral and cellular immune responses of calves exposed to a single dose of Bovela(®) bovine viral diarrhea virus (BVDV) live double deleted vaccine or a field strain virus (FSV) of BVDV type 2 (strain 890). Thirty seronegative, colostrum-deprived 5 month-old Holstein steer calves that tested negative for persistent BVDV by ear notch immunohistochemistry and seronegative to BVDV types 1 and 2 were used. Calves were screened by multi-parameter flow cytometry (MP-FCM) 1 week before vaccination to ensure that they were negative for T cell responses to the BVDV types 1 and 2 viruses in the Bovela(®) vaccine. Calves were assigned to 3 treatment groups: control (PBS), FSV inoculated, and Bovela(®) vaccinated. The humoral response was tested by standard serum virus neutralization (SVN) test to BVDV types 1 (Singer strain) and 2 (strain 125). The response by CD4, CD8, and gamma delta (γδ TCR) T cells was evaluated by MP-FCM using individual BVDV types 1 and 2 from Bovela(®) vaccine as recall antigens at 5, 6, and 7 weeks after vaccination. Activation markers used were upregulation of surface CD25 (IL-2R), intracellular interferon gamma (IFNγ) and intracellular interleukin 4 (...Continue Reading

Citations

Mar 9, 2018·Pathogens·Volker Moennig, Paul Becher
Nov 12, 2018·Transboundary and Emerging Diseases·Caitlin A EvansM Carolyn Gates
Dec 8, 2020·Frontiers in Immunology·Neha SangewarWaithaka Mwangi
Mar 26, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Neha SangewarWaithaka Mwangi
Jun 1, 2021·Frontiers in Veterinary Science·Anwar A G Al-KubatiMaged Gomaa Hemida
Jun 19, 2021·Frontiers in Veterinary Science·David GrahamMaria Guelbenzu

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