PMID: 6411463Jun 1, 1983Paper

Comparison of immunodiffusion and crossed-immunoelectrophoresis in the diagnosis of invasive candidiasis

European Journal of Clinical Microbiology
A N VanmaliB Z Ngwenya

Abstract

A retrospective single blind study was conducted to compare the efficacy of crossed-immunoelectrophoresis using concanavalin A intermediate gel with double immunodiffusion tests in the diagnosis of invasive candidiasis. On the basis of cytoplasmic antibody detection, crossed-immunoelectrophoresis with concanavalin A differentiated reliably between Candida albicans fungemia and invasive candida infection, whereas double immunodiffusion did not. With regard to sensitivity, specificity, and predictive value crossed-immunoelectrophoresis with concanavalin A was superior to double immunodiffusion. Analysis of double immunodiffusion precipitin bands revealed fuzzy and sharp precipitin bands which corresponded to mannan (fuzzy) and cytoplasmic (sharp) antibodies in the crossed-immunoelectrophoresis with concanavalin A assay. Therefore, the finding of sharp bands in the double immunodiffusion procedure supports the diagnosis of invasive candidiasis.

References

Nov 1, 1975·Infection and Immunity·R E SyversonC C Campbell
Nov 1, 1978·American Journal of Clinical Pathology·R E SyversonJ R Gibian
Aug 1, 1975·Archives of Internal Medicine·E D EverettT C Eickhoff
Jul 7, 1975·JAMA : the Journal of the American Medical Association·A F KriegR S Galen
Feb 1, 1972·American Journal of Clinical Pathology·C L TaschdjianE F Toni
Aug 1, 1972·Journal of Medical Microbiology·V C StanleyC J Carroll
Jan 1, 1973·American Journal of Clinical Pathology·C T Dolan, R P Stried
Feb 1, 1972·The Journal of Infectious Diseases·E Goldstein, P D Hoeprich
Jun 1, 1969·Sabouraudia·P J KozinnA Teitler
Nov 4, 1969·Journal of Medical Microbiology·I G MurrayG C Turner
Apr 1, 1967·The Journal of Infectious Diseases·C L TaschdjianM A Halle
Sep 1, 1967·Annals of Internal Medicine·C A Ellis, M L Spivack
Jan 1, 1964·The Journal of Pathology and Bacteriology·F C STALLYBRASS

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