Comparison of immunofluorescence assay and multiplexed microparticle-based immunoassay for detecting Epstein-Barr virus viral capsid antigen antibodies

Journal of Virological Methods
Aysin ZeytinogluRuchan Yazan Sertoz

Abstract

A new multiplexed microparticle-based immunoassay was compared with the immunofluorescence assay that is used widely for detecting EBV-specific antibodies in immunocompetent patients. Serum samples of 162 patients submitted for routine EBV diagnosis were tested for viral capsid antigen IgM, viral capsid antigen IgG and serological profile interpretations with both systems. The result concordances were 94.2%, 93.6%, and 92.1%, respectively. Multiplexed microparticle-based immunoassay can be an alternative to immunofluorescence assay especially in laboratories receiving large numbers of samples.

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Citations

Aug 7, 2009·Journal of Clinical Microbiology·J S KluttsA M Gronowski
Jun 17, 2008·The Journal of Molecular Diagnostics : JMD·Margaret L Gulley, Weihua Tang
Mar 12, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·O Devanthéry, P Meylan

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