Comparison of immunofluorescence, Western blotting, and cross-adsorption assays for diagnosis of African tick bite fever

Clinical and Diagnostic Laboratory Immunology
Mogens JenseniusDidier Raoult

Abstract

In testing paired serum samples from 40 consecutive cases of African tick bite fever, we detected diagnostic antibodies against spotted fever group rickettsiae in 45% of the patients by immunofluorescence assay (IFA) and in 100% of the patients by Western blotting (WB) (P < 0.01). A specific diagnosis of Rickettsia africae infection could be established in 15% of the patients by IFA and in 73% of the patients by a combination of WB and cross-adsorption assays (P < 0.01).

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Citations

Jun 19, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Nathalie RochMax Maurin
Aug 4, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Malavika PrabhuJohn A Crump
Nov 19, 2008·PLoS Neglected Tropical Diseases·Philippe ParolaDidier Raoult
Sep 22, 2010·PLoS Neglected Tropical Diseases·Oleg MediannikovDidier Raoult
Feb 24, 2006·Emerging Infectious Diseases·Patrick J Kelly
Mar 1, 2007·Emerging Infectious Diseases·Patrick KellyEdward B Breitschwerdt
Nov 4, 2008·Emerging Infectious Diseases·Nora MokraniDidier Raoult
Nov 7, 2009·Emerging Infectious Diseases·Mogens JenseniusUNKNOWN GeoSentinel Surveillance Network
Feb 5, 2011·The American Journal of Tropical Medicine and Hygiene·Emily BeelerMarina E Eremeeva
Mar 18, 2010·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Fabrice AlthausBlaise Genton
Dec 1, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·N BoudebouchD Raoult
Sep 5, 2014·Journal of Clinical Microbiology·Meagan F VaughnSteven R Meshnick
Sep 14, 2007·Scandinavian Journal of Infectious Diseases·Karin ElfvingKenneth Nilsson
Apr 14, 2015·Future Microbiology·Alison Luce-FedrowAllen L Richards
Mar 30, 2016·The American Journal of Tropical Medicine and Hygiene·Josie DelisleAbelardo C Moncayo

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