Comparison of immunogenicities of recombinant Plasmodium vivax merozoite surface protein 1 19- and 42-kiloDalton fragments expressed in Escherichia coli

Infection and Immunity
Suraksha SachdevaVirander S Chauhan

Abstract

The 42- and 19-kDa C-terminal fragments of merozoite surface protein 1 (MSP-1(42) and MSP-1(19), respectively) are both promising blood-stage vaccine candidate antigens. At present, it is not clear which of the two antigens will be more suitable for inclusion in a cocktail malaria vaccine. In the present study, we expressed the two C-terminal fragments of Plasmodium vivax MSP-1 (PvMSP-1) in an Escherichia coli expression system and purified them by using a rapid two-step protocol. Both of the products were recognized by monoclonal antibodies against PvMSP-1 as well as by immune sera from several individuals exposed to P. vivax. We analyzed and compared the immunological responses to recombinant PvMSP-1(19) and PvMSP-1(42) in mice by using six different adjuvant formulations. Moderate to high antibody responses were observed with both of the antigens in different adjuvant formulations. Surprisingly, alum, which is generally considered to be a poor adjuvant for recombinant malaria antigens, was found to be as good an adjuvant as Montanide ISA 720, ASO2A, and other adjuvant formulations. Most adjuvant formulations induced high levels of immunoglobulin G1 (IgG1), followed by IgG3 and IgG2. Lymphocytes from animals in the PvMSP-1(42...Continue Reading

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Citations

Jul 9, 2013·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Dilip PawarK S Jaganathan
Jan 24, 2007·Infection and Immunity·Amit KumarVirander Singh Chauhan
Sep 10, 2014·Protein Expression and Purification·Inayat Hussain SheikhNuzhat A Kaushal
Apr 16, 2009·International Journal for Parasitology·Thilan WickramarachchiAsif Mohmmed
Nov 13, 2007·Veterinary Immunology and Immunopathology·Lidija HabjanecJelka Tomasić
Nov 5, 2013·Clinical & Developmental Immunology·Fernanda G VersianiPaulo A Nogueira
Mar 9, 2007·Molecular and Biochemical Parasitology·Jeffrey J BabonAnthony A Holder
Oct 3, 2006·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·M Andreina PachecoAnanias A Escalante
Nov 24, 2007·International Journal for Parasitology·Manzar J HossainPawan Malhotra
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Nov 18, 2005·The American Journal of Tropical Medicine and Hygiene·Alejandro Jordan-VillegasSócrates Herrera

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