Comparison of immunologic assays for detecting immune responses in HIV immunotherapeutic studies: AIDS Clinical Trials Group Trial A5181.

Clinical and Vaccine Immunology : CVI
Bernard J C MacatangayR Pat Bucy

Abstract

This study was designed to evaluate which of several T-cell-specific, immune response assays are the most relevant in measuring the key characteristics of an effective immune response to HIV-1. Using 5 HIV-1 antigens as stimulants, we assessed lymphocyte proliferation, supernatant gamma interferon (IFN-gamma) cytokine production (CP), single-cell IFN-gamma production by enzyme-linked immunospot (ELISPOT) assay, with and without Epstein-Barr virus-transformed B-lymphoblastoid cell lines (B-LCLs), and intracellular cytokine production (ICC) for IFN-gamma and interleukin 2 (IL-2) by flow cytometry. We used these to compare specimens from HIV-1-infected subjects who were virally suppressed with a stable antiretroviral therapy (ART) regimen (group A) with specimens from subjects not on ART but with HIV-1 viremia of <3,000 copies/ml (group B). The lymphocyte proliferation assay (LPA) did not significantly differentiate between the two groups. Using fresh peripheral blood mononuclear cells (PBMCs), the CP and ELISPOT assays for IFN-gamma detected the greatest differences between the two groups, specific for three of the five HIV-1 antigens, whereas significant differences were seen only in response to one antigen when cryopreserved ce...Continue Reading

Associated Clinical Trials

References

Jun 1, 2001·Current Infectious Disease Reports·R. Pat Bucy
Jul 27, 2001·Journal of Immunological Methods·H T MaeckerL J Picker
Sep 4, 2004·Journal of Immunological Methods·S JanetzkiK S Panageas
Mar 8, 2008·Nature Reviews. Immunology·Robert A SederMario Roederer
Mar 28, 2008·Journal of Leukocyte Biology·Xiao-Li HuangCharles R Rinaldo
Apr 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sandra A CalarotaFranco Lori
Jun 11, 2008·Critical Reviews in Immunology·Shinichiro FuseEdward J Usherwood
Dec 10, 2008·Expert Opinion on Biological Therapy·B M BakerB D Walker
Dec 20, 2008·Journal of Internal Medicine·C R Rinaldo
Mar 14, 2009·Nature Protocols·Hendrik StreeckBruce D Walker
Nov 6, 2009·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Jean-Pierre RoutyRafick-Pierre Sékaly

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