Comparison of Immunomodulation Properties of Porcine Mesenchymal Stromal/Stem Cells Derived from the Bone Marrow, Adipose Tissue, and Dermal Skin Tissue

Stem Cells International
Sun-A OckGyu-Jin Rho

Abstract

Mesenchymal stromal/stem cells (MSCs) demonstrate immunomodulation capacity that has been implicated in the reduction of graft-versus-host disease. Accordingly, we herein investigated the capacity of MSCs derived from several tissue sources to modulate both proinflammatory (interferon [IFN] γ and tumor necrosis factor [TNF] α) and immunosuppressive cytokines (transforming growth factor [TGF] β and interleukin [IL] 10) employing xenogeneic human MSC-mixed lymphocyte reaction (MLR) test. Bone marrow-derived MSCs showed higher self-renewal capacity with relatively slow proliferation rate in contrast to adipose-derived MSCs which displayed higher proliferation rate. Except for the lipoprotein gene, there were no marked changes in osteogenesis- and adipogenesis-related genes following in vitro differentiation; however, the histological marker analysis revealed that adipose MSCs could be differentiated into both adipose and bone tissue. TGFβ and IL10 were detected in adipose MSCs and bone marrow MSCs, respectively. However, skin-derived MSCs expressed both IFNγ and IL10, which may render them sensitive to immunomodulation. The xenogeneic human MLR test revealed that MSCs had a partial immunomodulation capacity, as proliferation of ac...Continue Reading

References

Apr 2, 1999·Science·M F PittengerD R Marshak
Jul 18, 2002·The Annals of Thoracic Surgery·Takayuki SaitoRay C J Chiu
Jan 9, 2003·Lancet·Christof StammGustav Steinhoff
May 24, 2003·Leukemia·S ZimmermannU M Martens
May 10, 2005·Journal of Cellular Physiology·Victor VacantiTechung Lee
Jul 20, 2005·Bulletin of Experimental Biology and Medicine·R A MusinaG T Sukhikh
Oct 11, 2005·Experimental and Molecular Pathology·Wei ZhuYongchang Chen
Nov 29, 2005·Ageing Research Reviews·Sebastian SetheAlexandra Stolzing
Apr 3, 2007·Trends in Immunology·Antonio UccelliLorenzo Moretta
Apr 13, 2007·Developmental Biology·Yifei LiuLin Liu
Jun 16, 2007·In Vitro Cellular & Developmental Biology. Animal·Chang-qing QuGong-she Yang
Apr 1, 2008·Transplantation Proceedings·L L WeiY R Lu
Apr 9, 2008·Stem Cells and Development·Tatyana A ProkhorovaMoustapha Kassem
May 13, 2008·Lancet·Katarina Le BlancUNKNOWN Developmental Committee of the European Group for Blood and Marrow Transplantation
Jul 3, 2008·Methods : a Companion to Methods in Enzymology·Bruce A BunnellCynthia Ripoll
Nov 22, 2008·The Journal of Pathology·A I Caplan
Feb 11, 2009·Cell·Jeong Beom KimHans R Schöler
Jul 1, 2009·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·W GeH Wang
May 27, 2011·Veterinary Research Communications·Oksana RaabeStefan Arnhold
Oct 4, 2011·International Journal of Oncology·Ariane GrothIngrid Herr
Nov 15, 2011·Journal of Tissue Engineering and Regenerative Medicine·Eun-Ju KangGyu-Jin Rho
Jun 19, 2012·Cell Stem Cell·Armand Keating
Jul 11, 2012·Cell Transplantation·Noélle D GermainLaura B Grabel

❮ Previous
Next ❯

Citations

Sep 13, 2019·Cell Transplantation·Yu YouZuo-Jin Liu
Jul 4, 2020·Journal of Cellular Physiology·Alexandra CalleMiguel Ángel Ramírez
Nov 20, 2020·Scientific Reports·Joanna BukowskaBarbara Gawrońska-Kozak
Jun 1, 2021·Journal of Burn Care & Research : Official Publication of the American Burn Association·Tiffany C HeardDavid M Burmeister

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
PCR
electrophoresis
Protein Assay
FACS
enzyme-linked immunosorbent assays
flow cytometry

Software Mentioned

SPSS
BD FACS software

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.