PMID: 25752142Mar 11, 2015Paper

Comparison of immunoprotection between vaccination with meq-deleted Marek's disease virus and vaccine strain CVI988/Rispens

Wei sheng wu xue bao = Acta microbiologica Sinica
Luntao DuanZhi-zhong Cui

Abstract

To evaluate and compare the immunoprotection between a meq-deleted Marek's disease virus (MDV) and CVI988/Rispens against MDV very virulent strain GX0101. In total 120 one-day-old SPF chickens were divided into 4 groups (30 each) and kept in 5 isolators with positive pressure-filtered air. At 1 day of age, 2000 PFU of SC9-1 was inoculated subcutaneously into each bird in group 1; 2000 PFU of commercial vaccine CVI988/ Rispens was inoculated subcutaneously into each bird in group 2. No viral challenge was made in group 3 and 4 as controls. Five days later chickens in group 1, 2, 3 were challenged intra-abdominally with 2000 PFU of very virulent MDV strain GX0101. During 90 days after challenge, all dead birds were recorded and checked for necropsy. The tumor-suspected tissues were examined by histopathological biopsy. The antibody titers induced by AIV and NDV vaccination and propagation dynamics of MDV GX0101 were detected. At the same time, parallel tests were performed on Hy-Line Brown chickens containing MDV maternal antibody. SC9-1 stain provided 100% protective efficiency against very virulent GX0101 challenge in SPF and Hy-Line Brown chickens. CVI988/Rispens provided 86. 7% protective efficiency against very virulent GX01...Continue Reading