PMID: 6268652Sep 1, 1981Paper

Comparison of immunosorbent electron microscopy, enzyme immunoassay and counterimmunoelectrophoresis for detection of human rotavirus in stools

Journal of Virological Methods
G ObertM H van Regenmortel

Abstract

The detection of human rotaviruses by routine electron microscopy examination of stool specimens has been compared with the sensitivity of detection obtainable by three different immunoassays. These assays are: 1) immunosorbent electron microscopy (ISEM), which consists of the serological trapping of viruses on electron microscopy grids coated with protein A and specific viral antiserum; 2) an enzyme-linked immunosorbent assay (ELISA), in which the primary antibody is rabbit anti-rotavirus immunoglobulin, the secondary antibody is chicken anti-rotavirus immunoglobulin extracted from egg yolk of immunized hens, and the indicator antibody is alkaline phosphatase-conjugated rabbit anti-chicken immunoglobulin; 3) counterimmunoelectrophoresis (CIE). A total of 63 stool specimens from infants with gastroenteritis were examined. Of these, 23 and 24 specimens were found to contain rotavirus by electron microscopy and CIE, respectively. When scored by ELISA and ISEM, 37 and 39 were found to be positive, respectively. Confirmatory inhibition assays were necessary to eliminate some false positive reactions in ELISA. Detection of human rotaviruses in stools by ISEM is as sensitive as by ELISA, but in weakly positive specimens, ISEM offers ...Continue Reading

References

Jul 1, 1978·The Journal of General Virology·M S McNulty
Nov 1, 1977·Journal of Clinical Microbiology·D J Ellens, P W de Leeuw
Sep 1, 1979·Journal of Clinical Microbiology·R H Yolken, P J Stopa
May 1, 1977·Infection and Immunity·P J MiddletonJ S Tam
Jan 1, 1980·Journal of Clinical Microbiology·G Zissis, J P Lambert
Jul 1, 1980·Journal of Clinical Microbiology·A NicolaieffM H van Regenmortel
Jan 1, 1980·Immunological Communications·A PolsonM H van Regenmortel
Jun 1, 1973·American Journal of Public Health

❮ Previous
Next ❯

Citations

Apr 1, 1982·Journal of Virological Methods·A NicolaieffM H Van Regenmortel
Jul 1, 1985·Journal of Virological Methods·M H Van Regenmortel, J Burckard
Aug 1, 1986·Journal of Virological Methods·B A HeinzG L Hehl
Jan 14, 1999·Journal of Veterinary Pharmacology and Therapeutics·B RanheimN E Søli
Jun 1, 1990·Journal of Veterinary Pharmacology and Therapeutics·A E Waterman, W Kalthum
Sep 12, 2013·PloS One·Claudio F LanataUNKNOWN Child Health Epidemiology Reference Group of the World Health Organization and UNICEF
Feb 1, 1988·Journal of Clinical Microbiology·S L VonderfechtR H Yolken
Sep 1, 1984·Journal of Clinical Microbiology·J H HughesV V Hamparian
Feb 1, 1984·Journal of Clinical Microbiology·G GernaE Percivalle

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.