PMID: 9439969Jan 24, 1998Paper

Comparison of immunostainings for proliferating cell nuclear antigen and Ki-67 in human extraocular lesions

Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie
T KodamaT Setogawa

Abstract

Cell kinetic information is helpful to understand disease progression, treatment response, and prognosis of the neoplasms. To compare the usefulness and limitations of antibodies that recognize the cell cycle-associated molecules, proliferating cell nuclear antigen (PCNA) and Ki-67, we performed immunostaining in formalin-fixed, paraffin-embedded tissue sections of extraocular lesions. Specimens were obtained from patients undergoing routine surgical procedures. Formalin-fixed, paraffin-embedded sections were stained for PCNA and Ki-67 using the monoclonal antibodies PC10 and MIB-1, respectively. Microwave oven heating for antigen retrieval was performed before immunostaining. In squamous cell carcinomas and basal cell carcinomas, PCNA immunostaining varied greatly. Basal cells of benign epithelial lesions showed moderate to weak PCNA staining. Strong PCNA immunoreactivity was demonstrated in foci of inflammation and germinal centers. Microwave processing enhanced the intensity of those PCNA immunostainings. As the PCNA immunostaining intensity had a great variability, the absolute numbers of PCNA-positive cells were hard to count in some tissues. High Ki-67 counts were observed in squamous cell carcinomas, foci of inflammatory...Continue Reading

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Citations

Jun 6, 2000·Micron : the International Research and Review Journal for Microscopy·T Ezaki
Dec 10, 2008·Journal of Immunological Methods·Fabio D'AmicoFranca Stivala
Sep 26, 2003·Cornea·Surendra Basti, Marian S Macsai
Aug 26, 2006·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·S H KimH S Ryu
Dec 4, 2019·Indian Journal of Dermatology, Venereology and Leprology·Siva Rami Reddy KarumuriNaresh Babu Thambisetti

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