Comparison of in-house and commercial sample preparation and PCR amplification systems for detection of human immunodeficiency virus type 1 DNA in blood samples from Tanzanian adults.

Journal of Clinical Microbiology
E LyamuyaG Biberfeld

Abstract

This study compared the performance of several in-house nested PCR systems and the Amplicor human immunodeficiency virus type 1 (HIV-1) PCR kit in the detection of HIV-1 DNA in Tanzanian samples prepared by two different methods. All six of the in-house primer sets evaluated had a higher sensitivity for HIV DNA detection in samples prepared by the Amplicor PCR sample preparation method than in those prepared by the Ficoll-Isopaque (FIP) density gradient centrifugation method. A sensitivity of 100% was achieved by combining two in-house primer sets. The sensitivity of the standard Amplicor HIV-1 PCR kit was only 59%, whereas a modified Amplicor HIV-1 PCR test had a sensitivity of 98%. Our data show that Tanzanian samples prepared by the Amplicor preparation method are more suitable for HIV-1 PCR testing than samples prepared by the FIP method. The modified, but not the standard, Amplicor HIV-1 PCR kit provides an alternative to the nested in-house PCR technique for the diagnosis of HIV infection.

References

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Citations

Jun 6, 2000·Journal of Acquired Immune Deficiency Syndromes : JAIDS·W W FawziD Spiegelman
Nov 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anna-Lena SpetzJorma Hinkula
Jul 29, 2004·AIDS·Boris RenjifoUNKNOWN Tanzanian Vitamin and HIV Study Group
May 21, 1999·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·M V NilsenM A Sommerfelt
Jul 31, 2002·Transfusion·Rigmor ThorstenssonSören Andersson
Aug 15, 1997·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·N YoungT D Mastro
Oct 31, 2000·Scandinavian Journal of Infectious Diseases·A Alaeus

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