PMID: 11933274Apr 5, 2002Paper

Comparison of in-house polymerase chain reaction method with the Roche Amplicor technique for detection of Mycobacterium tuberculosis in cytological specimens

Diagnostic Cytopathology
Ravi MehrotraSue Kohlhepp

Abstract

Two techniques have been approved by the United States FDA for diagnosis of tuberculosis in smear positive sputa: LCX M. tuberculosis, a ligase chain reaction procedure manufactured by Abbott Laboratories, and Amplicor, a polymerase chain reaction (PCR) procedure manufactured by Roche. However, these commercial methods are expensive and beyond the reach of laboratories in most developing countries. We compared the Roche Amplicor kit with an in-house PCR using a primer set for Mycobacterium tuberculosis/bovis directed at MPB 64 protein gene. It was able to distinguish between M. tuberculosis, M. avium, M. gordonii, M. intracellularae, and M. kansasii. Fifty-seven cytological samples were submitted to the laboratory for molecular diagnosis of M. tuberculosis. Both procedures were run on every sample submitted and the two methods agreed completely. The custom-made method is less expensive than the commercial technique.

References

Feb 1, 1992·Acta Paediatrica·M NaritaH Kobayashi

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Citations

Jan 2, 2010·BMC Infectious Diseases·Luciene C SchererAfrânio L Kritski
Mar 18, 2009·Biochemical Society Transactions·Jim HuggettAlimuddin Zumla

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