PMID: 8111069Jan 1, 1993

Comparison of in situ hybridization and immunocytochemistry for the detection of residual beta cells in the pancreas of streptozotocin-treated diabetic rats

Acta Diabetologica
J Van GompelG Klöppel


The relative efficacy of immunocytochemistry versus in situ hybridization in identifying residual beta cells was studied in rats with streptozotocin-induced diabetes. Consecutive sections of pancreas of streptozotocin-treated diabetic rats and control animals were alternately subjected to in situ hybridization (synthetic oligonucleotides complementary to rat preproinsulin mRNA) and immunocytochemistry (monoclonal antibodies to insulin). The results obtained with both methods were quantitated with the use of computer-assisted image analysis, and the ratio of cells positive by immunocytochemistry to those positive by in situ hybridization was determined. Under normoglycaemic conditions the values obtained by immunocytochemistry correlated well with those obtained by in situ hybridization (immuno/in situ > 95%). In the streptozotocin diabetic animals, however, immunocytochemistry resulted in a distinct underestimation of the number of residual beta cells (immuno/in situ < 80%). This difference was even more striking in small islet cell clusters (< 100 microns) (immuno/in situ 20%). These results suggest that in situ hybridization for prohormone mRNA is the method of choice for the identification of residual or regenerating beta ce...Continue Reading


Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·L OrciR H Unger
Apr 1, 1991·The Journal of Pathology·Q HamidJ M Polak
Dec 1, 1987·Human Pathology·R V Lloyd
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·L ChenR H Unger
Sep 1, 1985·Endocrinology·D G PipeleersW Gepts
Sep 1, 1969·Diabetes·G Brosky, J LOGOTHETOPOULOS

Related Concepts

Monoclonal Antibodies
Monoclonal antibodies, antineoplastic
Benign Tumor of Pancreas
Insulin B Chain
Streptozotocin Diabetes
MUROMONAB-CD3, Parenteral,5 MG Administered

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