Comparison of in situ sequence type analysis of Legionella pneumophila in respiratory tract secretions and environmental samples of a hospital in East Jerusalem

Epidemiology and Infection
Lina JaberDina M Bitar

Abstract

Legionella pneumophila genotyping is important for epidemiological investigation of nosocomial and community-acquired outbreaks of legionellosis. The prevalence of legionellosis in pneumonia patients in the West Bank was monitored for the first time, and the sequence types (STs) from respiratory samples were compared with STs of environmental samples from different wards of the hospital. Sputum (n = 121) and bronchoalveolar lavage (BAL) (n = 74) specimens were cultured for L. pneumophila; genomic DNA was tested by 16S rRNA polymerase chain reaction (PCR) amplification. Nested PCR sequence-based typing (NPSBT) was implemented on DNA of the respiratory and environmental PCR-positive samples. Only one respiratory specimen was positive for L. pneumophila by culture. BAL gave a higher percentage of L. pneumophila-positive samples, 35% (26/74) than sputum, 15% (18/121) by PCR. NPSBT revealed the following STs: ST 1 (29%, 7/24), ST 461 (21%, 5/24), ST 1037 (4%, 1/24) from respiratory samples, STs from environmental samples: ST 1 (28.5%, 4/14), ST 187 (21.4%, 3/14) and ST 2070, ST 461, ST 1482 (7.1%, 1/14) each. This study emphasises the advantage of PCR over culture for the detection of L. pneumophila in countries where antibiotics ar...Continue Reading

References

Apr 2, 2003·The Journal of Antimicrobial Chemotherapy·Jorge Roig, Jordi Rello
Jul 5, 2003·Journal of Clinical Microbiology·Helena AurellSophie Jarraud
Nov 6, 2007·The Journal of Infection·B M W Diederen
Dec 7, 2007·Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre·J A QasemZ U Khan
Dec 21, 2007·Journal of Clinical Microbiology·Bram M W DiederenMarcel F Peeters
May 21, 2009·FEMS Microbiology Reviews·Wenjun LiPierre-Edouard Fournier
May 26, 2009·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Mireia Coscollá, Fernando González-Candelas
Nov 18, 2009·New South Wales Public Health Bulletin·Christopher C BlythSharon C A Chen
Jan 27, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M MentastiT G Harrison
Nov 15, 2012·Methods in Molecular Biology·Christian LückTimothy G Harrison
Oct 15, 2013·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J Moran-GiladUNKNOWN ESCMID Study Group for Legionella Infections (ESGLI)
Mar 1, 2014·Epidemiology and Infection·R M A GrahamA V Jennison
Jan 9, 2015·Clinical Microbiology Reviews·Jeffrey W Mercante, Jonas M Winchell
Apr 4, 2015·Jundishapur Journal of Microbiology·Nader Divan KhosroshahiPouran Johari
Apr 9, 2015·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M MentastiV J Chalker
Apr 13, 2015·Water Research·Sarah Rodríguez-MartínezMalka Halpern
Jul 24, 2015·Journal of Clinical Microbiology·Thijs BoschLeo M Schouls
Aug 21, 2015·Journal of Clinical Microbiology·Derrick J ChenSandra S Richter
Oct 9, 2016·Environmental Research·Luigi PrincipePaolo Visca
Feb 6, 2017·Infectious Disease Clinics of North America·Almudena BurilloEmilio Bouza
Oct 13, 2017·Central European Journal of Public Health·Dimosthenis ChochlakisAnna Psaroulaki

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