PMID: 3755682Jun 1, 1986Paper

Comparison of in vitro activity of cytotoxic drugs towards human carcinoma and leukaemia cell lines

European Journal of Cancer & Clinical Oncology
G J FinlayB C Baguley

Abstract

Eight human haematopoietic cell lines and four human carcinoma lines were used to compare the activity of a number of cytotoxic drugs including amsacrine, the amsacrine analogue CI-921, methotrexate, nitracrine, doxorubicin, daunorubicin and 5-fluorouracil. Activity was assessed by means of semiautomated microculture growth inhibition assays. Cell density of the non-adherent cell lines was measured using the technique of Mosmann (J Immunol Methods 1983, 65, 55-63), in which the dye thiazolyl blue (MTT) is metabolised to a dark blue formazan product. This technique gives similar results to those obtained by direct cell counting in an electronic cell counter, and when applied to some adherent cell lines gives similar results to those obtained by the methylene blue staining technique previously developed (Anal Biochem 1984, 139, 272-277). Both methylene blue and MTT methods were used to investigate cytotoxicity in conjunction with semi-automated 96-well microculture plate techniques. The results show that the three T-cell leukaemia lines (CCRF-CEM, Jurkat and MOLT-4) are more sensitive to DNA-binding drugs (excluding nitracrine) than are the colon carcinoma lines (HCT-8, HT-29, SW480 and SW620). The more resistant haematopoietic l...Continue Reading

References

Jul 1, 1976·Cell and Tissue Kinetics·D W Ross

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Citations

Jan 1, 1994·Cancer Chemotherapy and Pharmacology·G J FinlayB C Baguley
Dec 2, 2005·Breast Cancer Research and Treatment·Dimitrios T P TrafalisCharalambos Camoutsis
Feb 1, 1989·International Journal for Parasitology·J C ComleyD C Jenkins
Oct 1, 1990·Mutation Research·L R FergusonW A Denny
Jan 1, 1995·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·U KöhlA Weissflog
Nov 1, 1995·Clinical and Experimental Pharmacology & Physiology·B C Baguley, G J Finlay
Apr 15, 2014·Phytochemistry·David PertuitMarie-Aleth Lacaille-Dubois
Feb 1, 2008·Expert Opinion on Drug Discovery·Bruce C Baguley, Elaine S Marshall
Apr 1, 1989·International Journal of Radiation Oncology, Biology, Physics·M E WattsM Woodcock
Jan 26, 2005·British Journal of Haematology·Dimitrios T P TrafalisAthanasios Papageorgiou
Jul 1, 1996·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·A C WilsonB C Baguley
Jan 1, 1995·Journal of Bioluminescence and Chemiluminescence·R D PettyI A Cree
Apr 3, 1999·British Journal of Cancer·F OroszJ Ovádi
Aug 19, 1993·International Journal of Cancer. Journal International Du Cancer·K PillweinP Chiba
Nov 8, 2017·Future Medicinal Chemistry·Savvas ThysiadisVasiliki Sarli
Jul 15, 1990·International Journal of Cancer. Journal International Du Cancer·C M TsaiB S Kramer
Apr 11, 2017·Frontiers in Pharmacology·Nikoletta KalenderoglouKaren L Wright

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