PMID: 6401805Feb 1, 1983Paper

Comparison of in vitro and in vivo measurements of dietary Ca exchangeability and bioavailability

The Journal of Nutrition
E M Wien, R Schwartz

Abstract

An in vitro method for measuring dietary mineral exchangeability (miscibility with an extrinsic isotopic tracer) was tested by comparison with in vivo measurements in rats. Collards, spinach and soybeans, intrinsically labeled with 45Ca, were fed to rats together with extrinsically added 47Ca. Absorption of both tracers was determined by measuring their concentrations in the femur 2 days after consumption of the labeled test meals. The same intrinsically and extrinsically labeled foods were digested in vitro for estimation of Ca exchangeability and solubility after peptic-pancreatic digestion. Corresponding in vivo and in vitro estimates of exchangeability agreed closely for the three foods tested. Solubility after in vitro peptic-pancreatic digestion (potential bioavailability) showed discrepancies. In vitro values were somewhat higher for collards than in vivo fractional absorption. The reverse was true for Ca in soybeans and spinach. Thus, the in vitro procedure appears adequate for measuring intrinsic dietary Ca exchangeability but requires modification if it is to be a reliable substitute for in vivo measurements of Ca bioavailability.

Citations

May 3, 2005·Annual Review of Plant Biology·Vincent R Franceschi, Paul A Nakata
Mar 1, 1986·The British Journal of Nutrition·A Ekanayake, P E Nelson
Dec 1, 1992·Zeitschrift für Ernährungswissenschaft·H TsuchitaT Yoshida
Jul 1, 2006·Functional Plant Biology : FPB·Paul A Nakata, Michele M McConn

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