PMID: 9639698Jun 26, 1998Paper

Comparison of in vivo mutagenesis in the endogenous Hprt gene and the lacI transgene of Big Blue(R) rats treated with 7, 12-dimethylbenz[a]anthracene

Mutation Research
M G ManjanathaD Casciano

Abstract

The lacI transgene of Big Blue(R) (BB) rats was evaluated as a reporter of in vivo mutation by comparing mutant frequencies (MFs) in it and in the endogenous Hprt gene. Seven-week old female BB rats were given single doses of 0, 20, 75 and 130 mg/kg of 7, 12-dimethylbenz(a)anthracene (DMBA) by gavage, and Hprt and lacI MFs in splenic lymphocytes were measured over a period of 18 weeks. The Hprt MFs in treated rats increased for 10 weeks and then declined; 130 mg/kg of DMBA produced a maximum Hprt MF of 168+/-11.4x10-6 clonable lymphocytes, while the MF in control rats was 7.4+/-1. 5x10-6. DMBA exposure of generic F344 rats resulted in a similar time-course of mutant induction but produced about 50% higher Hprt MFs with the 75 and 130 mg/kg doses. In contrast, the lacI MFs increased for 6 weeks and then remained relatively constant; 130 mg/kg of DMBA produced a maximum increase in lacI MF of 341+/-83x10-6 PFU compared with 25+/-5x10-6 PFU in control rats. The Hprt mutant frequencies in DMBA-treated BB and F344 rats were significantly increased over control values for every dose-time combination examined, while only the 130 mg/kg dose consistently produced lacI MFs that were significantly above the controls. In addition, the fold...Continue Reading

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Citations

Oct 11, 2003·Mutation Research·Véronique ThybaudNobuhiro Yajima
Dec 13, 2000·Mutation Research·T NohmiK Masumura
Dec 13, 2000·Mutation Research·J T MacGregorL Müller
Feb 25, 1999·Mutation Research·V N DobrovolskyR H Heflich
Jan 1, 2007·African Journal of Traditional, Complementary, and Alternative Medicines : AJTCAM·Subramanian BalakrishnanKasinathan Rajalingam
Mar 5, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Nan MeiRobert H Heflich
May 14, 2004·Environmental and Molecular Mutagenesis·Dale M WalkerVernon E Walker
Jun 26, 2001·Environmental and Molecular Mutagenesis·R R SwigerJ A Heddle
Mar 29, 2000·Environmental and Molecular Mutagenesis·J A HeddleN Yajima
Feb 22, 2008·Environmental and Molecular Mutagenesis·Steven M BryceStephen D Dertinger
Aug 30, 2008·Journal of Biochemical and Molecular Toxicology·K PanjamurthyN Senthil
Mar 19, 2020·Environmental and Molecular Mutagenesis·Bas-Jan van der LeedeJacky Van Gompel
Jun 3, 2015·Environmental and Molecular Mutagenesis·Vasily N DobrovolskyHaixia Lin
Feb 9, 2017·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Takehiko NohmiNaomi Toyoda-Hokaiwado
Jan 1, 2015·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Zhuhong ZhangNan Mei
Jan 11, 2001·Environmental and Molecular Mutagenesis·B H RihnG Keith
Mar 14, 2021·Environmental Pollution·Francisco Carlos da Silva JuniorSilvia Regina Batistuzzo de Medeiros
Aug 6, 2005·Mutation Research·Iain B LambertGeorge R Douglas

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