Comparison of inexperienced operators and experts in γH2A.X and 53BP1 foci assay for high-throughput biodosimetry approaches in a mass casualty incident.

International Journal of Radiation Biology
Martin BucherMaria Gomolka

Abstract

In case of population exposure by ionizing radiation, a fast and reliable dose assessment of exposed and non-exposed individuals is crucial important. In initial triage, physicians have to take fast decisions whom to treat with adequate medical care. In addition, worries about significant exposure can be taken away from hundreds to thousands non- or low exposed individuals. Studies have shown that the γH2A.X radiation-induced foci assay is a promising test for fast triage decisions. However, in a large-scale scenario most biodosimetry laboratories will quickly reach their capacity limit. The aim of this study was to evaluate the benefit of inexperienced experimenters to speed up the foci assay and manual foci scoring. The participants of two training courses performed the radiation-induced foci assay (γH2A.X) under the guidance of experts and scored foci (γH2A.X and 53BP1) on sham-irradiated and irradiated blood samples (0.05-1.5 Gy). The outcome of laboratory experiments and manual foci scoring by 26 operators with basic experience in laboratory work was statistically analyzed in comparison to the results from experts. Inexperienced operators prepared slides with significant dose-effects (0, 0.1 and 1.0 Gy) for semi-automatic ...Continue Reading

References

Apr 16, 1998·The Journal of Biological Chemistry·E P RogakouW M Bonner
Sep 9, 1999·The Journal of Cell Biology·E P RogakouW M Bonner
Oct 20, 2006·International Journal of Radiation Biology·Francesco MarchettiAndrew J Wyrobek
Jun 24, 2008·International Journal of Radiation Oncology, Biology, Physics·Sunirmal Paul, Sally A Amundson
Jan 13, 2010·Health Physics·Guy GartyDavid J Brenner
May 12, 2011·International Journal of Radiation Biology·Guy GartyDavid J Brenner
Aug 28, 2012·Radiation Protection Dosimetry·U KulkaA Wojcik
Jul 19, 2013·Radiation Research·K RothkammM Abend
Jul 26, 2013·Radiation Research·K RothkammM Abend
Aug 2, 2013·International Journal of Molecular Sciences·Laia HernándezAnna Genescà
Dec 3, 2013·International Journal of Radiation Biology·Elizabeth A AinsburyAndrzej Wojcik
Dec 12, 2013·Nature Reviews. Molecular Cell Biology·Stephanie Panier, Simon J Boulton
Jan 31, 2014·Radiation and Environmental Biophysics·Helen C TurnerG Garty
Feb 13, 2014·Radiation and Environmental Biophysics·Harold M SwartzAnn Barry Flood
Jan 27, 2015·Toxicological Sciences : an Official Journal of the Society of Toxicology·María Sánchez-FloresVanessa Valdiglesias
May 6, 2015·Microscopy Research and Technique·Guy GartyDavid J Brenner
Aug 6, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Matus DurdikIgor Belyaev
Aug 26, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Raavi VenkateswarluPerumal Venkatachalam
Oct 7, 2016·International Journal of Radiation Biology·Ulrike KulkaAndrzej Wojcik
Oct 26, 2016·International Journal of Radiation Biology·Andrzej WojcikUlrike Kulka
May 30, 2019·International Journal of Radiation Biology·Maciej GałeckiBeata Brzozowska-Wardecka

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.