Comparison of infectivity and spread between HSV-1 and HSV-2 based oncolytic viruses on tumor cells with different receptor expression profiles

Oncotarget
Xinping FuXiaoliu Zhang

Abstract

Herpes simplex virus (HSV) is one of the many viruses that have been modified or adapted for oncolytic purposes. There are two serotypes of HSV, HSV-1 and HSV-2. The majority of oncolytic HSVs, including T-VEC which has recently been approved by the US Food and Drug Administration (FDA) for clinical use in treating late stage melanoma patients, are derived from HSV-1. Recently, we and others have developed several HSV-2 based oncolytic viruses. During our in vitro characterization of oncolytic viruses developed from both serotypes (Baco-1 from HSV-1 and FusOn-H2 from HSV-2), we noticed there is a subpopulation of cancer cells in which both viruses could infect but only FusOn-H2 could spread from cell to cell on monolayers. This observation prompted us to investigate the virus receptor expression profiles in these and other tumor cells. Our data show the following: 1) This subpopulation of tumor cells only express nectin-2, not the other two major receptors (HVEM or nectin-1). 2) Baco-1 grows to a higher titer than FusOn-H2 in this subpopulation of tumor cells, but the latter kills these tumor cells more efficiently than the former. 3) FusOn-H2 is effective at treating tumors formed from these tumor cells while Baco-1 is complet...Continue Reading

References

Jun 6, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Xinping FuXiaoliu Zhang
Apr 2, 2004·Cellular Microbiology·Patricia G Spear
May 4, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Mikihito NakamoriXiaoliu Zhang
Dec 20, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Wendong LiZhihua Liu
Mar 30, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Xinping FuXiaoliu Zhang
May 19, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Xinping FuXiaoliu Zhang

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Citations

Sep 28, 2018·Biomedicines·Daria S ChulpanovaAlbert A Rizvanov

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Methods Mentioned

BETA
flow cytometry
Flow

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