PMID: 9444947Jan 28, 1998Paper

Comparison of infrared spectra of CLL cells with their ex vivo sensitivity (MTT assay) to chlorambucil and cladribine

Leukemia Research
K Z LiuHenry H Mantsch

Abstract

The drug resistance of leukemic cells from 21 patients with chronic lymphocytic leukemia (CLL) to the alkylating agent chlorambucil (CLB) and the nucleoside analog cladribine or 2-chlorodeoxyadenosine (CdA) was investigated by infrared spectroscopy. Drug sensitivities, determined in vitro with the tetrazolium dye (MTT) assay, were correlated with the infrared spectra of the CLL cells, applying linear discriminant analysis (LDA). The 63 spectra (three from each of the 21 samples), obtained before drug exposure, were successfully partitioned into drug-sensitive and drug-resistant groups; the LDA-based ex vivo prediction of the sensitivity to CdA or CLB was 85.7% and 80.3%, respectively. Similar changes in the composition/structure of DNA were observed between the spectra of the drug-sensitive and drug-resistant CLL cells for both CdA and CLB. However, CdA-resistant CLL cells could also be differentiated from CdA-sensitive CLL cells by spectral changes associated with membrane lipids; these differences were much less pronounced between CLB-resistant and CLB-sensitive CLL cells. We demonstrate here for the first time that infrared spectroscopy can be used as a new tool for predicting ex vivo drug response (sensitivity/resistance).

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Citations

Oct 11, 2007·Pharmacogenomics·David I EllisRoyston Goodacre
Dec 22, 1999·British Journal of Haematology·L JiaS M Kelsey
Jan 17, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T Chandramohan ReddyP Reddanna
Mar 27, 2010·Journal of Periodontal Research·X M XiangD A Scott
Mar 7, 2007·British Journal of Haematology·Kan-Zhi LiuDavid A Scott
Apr 1, 2005·Journal of Biomedical Materials Research. Part a·D GopinathR Jayakumar
Apr 2, 2008·Analytical and Bioanalytical Chemistry·Liqun Wang, Boris Mizaikoff
Jun 21, 2003·Clinical Chemistry·Kan-Zhi LiuHenry H Mantsch
May 29, 2009·The Analyst·Catherine KendallNicholas Stone

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