Abstract
The drug resistance of leukemic cells from 21 patients with chronic lymphocytic leukemia (CLL) to the alkylating agent chlorambucil (CLB) and the nucleoside analog cladribine or 2-chlorodeoxyadenosine (CdA) was investigated by infrared spectroscopy. Drug sensitivities, determined in vitro with the tetrazolium dye (MTT) assay, were correlated with the infrared spectra of the CLL cells, applying linear discriminant analysis (LDA). The 63 spectra (three from each of the 21 samples), obtained before drug exposure, were successfully partitioned into drug-sensitive and drug-resistant groups; the LDA-based ex vivo prediction of the sensitivity to CdA or CLB was 85.7% and 80.3%, respectively. Similar changes in the composition/structure of DNA were observed between the spectra of the drug-sensitive and drug-resistant CLL cells for both CdA and CLB. However, CdA-resistant CLL cells could also be differentiated from CdA-sensitive CLL cells by spectral changes associated with membrane lipids; these differences were much less pronounced between CLB-resistant and CLB-sensitive CLL cells. We demonstrate here for the first time that infrared spectroscopy can be used as a new tool for predicting ex vivo drug response (sensitivity/resistance).
References
Jan 1, 1976·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·K R Harrap, E W Gascoigne
Nov 1, 1990·The Journal of Clinical Investigation·C J CarreraD A Carson
Jan 1, 1991·Leukemia Research·A BegleiterJ B Johnston
Oct 1, 1990·Annals of Internal Medicine·K A FoonR P Gale
Jan 29, 1988·Biochimica Et Biophysica Acta·W K Surewicz, H H Mantsch
Jan 1, 1987·Pharmacology & Therapeutics·P B Farmer
Jan 1, 1985·Leukemia Research·E BenedettiG Spremolla
Mar 1, 1984·Cancer Treatment Reviews·S Somfai-RelleD T Vistica
Oct 1, 1994·The Journal of Experimental Medicine·M G CifoneR Testi
Aug 1, 1995·Leukemia Research·S MolicaL Levato
Feb 1, 1995·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·R L SomorjaiL Delbridge
May 1, 1995·Mutation Research·J BramsonL C Panasci
Jun 1, 1995·Leukemia & Lymphoma·N E KayL C Peterson
May 1, 1996·Nature Medicine·C FriesenK M Debatin
Apr 1, 1996·The Journal of Experimental Medicine·N ZamzamiG Kroemer
May 25, 1996·Lancet·S JohnsonW Hiddemann
Jul 12, 1996·Cell·X LiuX Wang
Jun 1, 1996·British Journal of Haematology·Y H MinY W Ko
Aug 1, 1996·Leukemia Research·C P SchultzH H Mantsch
Oct 1, 1996·Leukemia & Lymphoma·A BegleiterJ B Johnston
Apr 24, 1999·Leukemia·T RobakA B Skotnicki
Citations
Jun 24, 2003·The Journal of Laboratory and Clinical Medicine·Jagannathan RameshShaul Mordechai
Jun 10, 1998·Cancer Letters·K Z LiuH H Mantsch
Aug 25, 1999·British Journal of Haematology·A G BosanquetD G Oscier
Mar 23, 2013·Journal of Periodontology·Xiaoming XiangKan-Zhi Liu
Oct 11, 2007·Pharmacogenomics·David I EllisRoyston Goodacre
Dec 22, 1999·British Journal of Haematology·L JiaS M Kelsey
Jan 17, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T Chandramohan ReddyP Reddanna
Mar 27, 2010·Journal of Periodontal Research·X M XiangD A Scott
Aug 30, 2005·Blood Cells, Molecules & Diseases·Kan-Zhi LiuHenry H Mantsch
Mar 7, 2007·British Journal of Haematology·Kan-Zhi LiuDavid A Scott
Mar 17, 1999·American Journal of Obstetrics and Gynecology·K Z Liu, H H Mantsch
May 16, 2015·Neurobiology of Aging·Arpit MehrotraRajat Sandhir
Mar 8, 2003·Technology in Cancer Research & Treatment·Christian P Schultz
Oct 10, 2006·The Analyst·David I Ellis, Royston Goodacre
Apr 1, 2005·Journal of Biomedical Materials Research. Part a·D GopinathR Jayakumar
Apr 2, 2008·Analytical and Bioanalytical Chemistry·Liqun Wang, Boris Mizaikoff
Mar 24, 2009·The Analyst·Jaspreet BabrahNicholas Stone
Jun 21, 2003·Clinical Chemistry·Kan-Zhi LiuHenry H Mantsch
May 29, 2009·The Analyst·Catherine KendallNicholas Stone
Apr 20, 2018·Metabolic Brain Disease·Abhilasha SoodRajat Sandhir
Dec 3, 2020·Journal of Clinical Medicine·Weronika GonciarzMagdalena Chmiela