Comparison of inhibitory effects of polyanions on nitric oxide production by macrophages stimulated with LPS

Biological & Pharmaceutical Bulletin
Masaki OtsukaYukihiko Aramaki

Abstract

In this paper, we investigated the inhibitory mechanism of the production of nitric oxide (NO) by polyanions and liposomes composed of phosphatidylserine (PS-liposomes) focusing on cytokine production and mitogen activated protein kinase (MAP kinase) activation. NO production by macrophages was inhibited by treatment with oxidized lipoprotein (OxLDL), maleylated bovine serum albumin (mBSA), and heparin. No inhibitory effect was exhibited by poly-cytidilic acid (PolyC). To clarify the mechanism of the inhibitory effect of polyanions on NO production, we evaluated the productions of transforming growth factor-beta (TGF-beta) and interleukin (IL)-10 which are known to be anti-inflammatory cytokines. TGF-beta was produced when macrophages were treated with OxLDL as was the case with PS-liposomes. No increase in TGF-beta production was observed for mBSA, heparin, and PolyC. On the other hand, significant production of IL-10 was observed using mBSA. Extracellular signal-regulated kinase (ERK), a member of the MAP kinase superfamily, was activated when macrophages were treated with OxLDL as well as PS-liposomes. In the case of mBSA, the activation of ERK and c-Jun N-terminal kinase (JNK) was observed. No activation of p38 MAP kinase w...Continue Reading

References

Mar 7, 1996·Biochemical and Biophysical Research Communications·Y AramakiS Tsuchiya
Apr 1, 1997·Nature Medicine·L BenimetskayaC A Stein
Aug 28, 1997·Biochemical and Biophysical Research Communications·R MatsunoS Tsuchiya
Mar 26, 1998·Biochemical and Biophysical Research Communications·R MatsunoS Tsuchiya
Apr 2, 1999·European Journal of Biochemistry·D RhaindsL Brissette
Jun 23, 2000·Biological & Pharmaceutical Bulletin·T NakamuraJ Watanabe
May 23, 2002·The Journal of Biological Chemistry·Yuki KawasakiYoshinobu Nakanishi
Jul 29, 2004·Journal of Cellular Physiology·Michelle AlexanderMartin G Schwacha
Sep 3, 2004·Yonsei Medical Journal·Yong-Suk RyangJu-Youn Park
Sep 11, 2004·Biochemical and Biophysical Research Communications·Young-Hun YookHayyoung Lee
Oct 27, 2004·Biochemical and Biophysical Research Communications·Masaki OtsukaYukihiko Aramaki

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