PMID: 2505742Jul 1, 1989Paper

Comparison of inoculation regimes for the experimental production of swine erysipelas arthritis. II. Serological findings in a gel diffusion precipitin test and enzyme-linked immunosorbent assay

Australian Veterinary Journal
G J EamensP J Nicholls

Abstract

The serological response of pigs to Erysipelothrix rhusiopathiae inoculation was monitored by a gel diffusion precipitin test (GDPT) using a crude, serotype-specific, autoclaved antigen and an enzyme-linked immunosorbent assay (ELISA) using a heat-extracted, alcohol precipitated and molecular seived antigen previously shown to react with serum from pigs infected with serotypes 1 or 2. All pigs receiving 3 or 5 weekly intravenous inoculations of either a highly virulent (VRS 229) or a lowly virulent isolate (VRS 252) produced GDPT-reactive antibody within 3 weeks, but only 44% were still reactive at 8 to 9.5 weeks. The ELISA response was significantly higher in pigs inoculated with the highly virulent strain, and was similar in pigs receiving 3 or 5 doses of either strain. In a dose-response trial, after 3 doses of VRS 229, GDPT reactivity occurred earlier and was stronger in pigs given higher doses of E. rhusiopathiae, but the response peaked 3 to 5 weeks after the start of challenge and was short lived. GDPT reactivity correlated with dose, but not with the severity of arthritis. The ELISA demonstrated specific IgG antibody was present by 2 weeks, and persisted to at least 11 weeks. The ELISA reactivity was significantly highe...Continue Reading

References

Apr 27, 1971·Biochimica Et Biophysica Acta·J Curtis, F J Bourne
Oct 1, 1969·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·H D GeissingerP J Quinn
Apr 1, 1962·Journal of Comparative Pathology·K S CHODNIK, J W STEVENS

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