PMID: 9428790Jan 15, 1998Paper

Comparison of intestinal phospholipase A/lysophospholipase and sucrase-isomaltase genes suggest a common structure for enterocyte-specific promoters

DNA and Cell Biology
J K TaylorP G Traber

Abstract

Intestinal phospholipase A/lysophospholipase (IPAL) is an intestine-specific brush-border enzyme expressed during development and along the intestinal crypt-villus axis in a pattern similar to another well characterized brush-border enzyme, sucrase-isomaltase (SI). A tissue-specific DNase I hypersensitive site was identified in chromatin from intestinal nuclei immediately upstream from the transcriptional start site of the IPAL gene. Footprinting analysis showed that two DNA elements within the IPAL promoter were protected by intestinal nuclear proteins. The IPAL-FP1 element was shown to be a monomer binding site for Cdx1 and Cdx2, intestine-specific homeobox proteins. Moreover, this site was important for transcriptional activation of the promoter in intestinal cell lines via interaction with Cdx proteins. Nuclear proteins from both liver and intestine interacted with the IPAL-FP2 element, forming a complex consistent with binding to HNF1. Cdx and HNF1 binding sites have also been shown to be the two major regulatory elements responsible for transcriptional activation of the SI gene promoter, which directs intestine-specific transcription in transgenic mice. These findings suggest that enterocyte genes that are expressed in si...Continue Reading

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Citations

Sep 16, 2005·Cancer Chemotherapy and Pharmacology·Barbara A E de KoningAlexandra W C Einerhand
Apr 7, 2007·Digestive Diseases and Sciences·Barbara A E de KoningAlexandra W C Einerhand
Mar 22, 2005·Biochimica Et Biophysica Acta·Jesper T Troelsen
Sep 15, 2004·Toxicology and Applied Pharmacology·Philip A GregoryPeter I Mackenzie
Dec 6, 2002·Nucleic Acids Research·Mi-Sun HwangMi-Ae Yoo
Sep 19, 2009·BMC Gastroenterology·Mette BoydJesper T Troelsen
Jan 29, 2008·Scandinavian Journal of Gastroenterology·Jantine W P M van BaalKausilia K Krishnadath
Jan 31, 2008·Neoplasia : an International Journal for Oncology Research·Mary Ann S CrisseyJohn P Lynch
Sep 21, 2004·The Journal of Pathology·Emanuela PilozziLuigi Ruco
Apr 20, 2016·Frontiers in Genetics·Darius WlochowitzMehmet Gültas
Feb 24, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Matthew S KellerJohn P Lynch
Aug 15, 2003·World Journal of Gastroenterology : WJG·Zhou WangJing-Bo Sun
Jul 13, 2002·The Journal of Biological Chemistry·Cameron N JohnstoneJoan K Heath
Jan 13, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Joyce K DivineTheodore C Simon
Dec 4, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Mei-Lun WangGary D Wu
Dec 24, 2018·American Journal of Physiology. Gastrointestinal and Liver Physiology·Åke Nilsson, Rui-Dong Duan
Jul 7, 1999·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J N FreundI Duluc
Nov 24, 1999·The Journal of Biological Chemistry·F XuT Jin
Mar 19, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Fuad AlkhouryRichard A Hodin
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Dec 21, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·E SuhP G Traber
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Nov 12, 1998·Differentiation; Research in Biological Diversity·R KrauseR Fundele
Oct 21, 2000·Gastroenterology·D G SilbergP G Traber

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