Comparison of Intraoral Bone Regeneration with Iliac and Alveolar BMSCs

Journal of Dental Research
F WangD Zou

Abstract

This study compared the osteogenic potential of bone marrow mesenchymal stem cells (BMSCs) of iliac and alveolar origins (I-BMSCs and Al-BMSCs, respectively), which were transplanted in combination with β tricalcium phosphate (β-TCP) in peri-implant bone defects to investigate the osseointegration between dental implants and tissue-engineered bone in dogs. Specifically, I-BMSCs and Al-BMSCs were cultured, characterized, and seeded on β-TCP and subjected to immunoblotting analyses and alkaline phosphatase activity assays. Subsequently, these cell-seeded scaffolds were implanted into defects that were freshly generated in the mandibular premolar areas of 4 dogs. The defects were covered with β-TCP + Al-BMSCs ( n = 6), β-TCP + I-BMSCs ( n = 6), or β-TCP ( n = 6) or served as the blank control ( n = 6). After healing for 12 wk, the formation and mineralization of new bones were assessed through micro-computed tomographic, histologic, and histomorphometric analyses, and bone-to-implant contacts were measured in the specimens. It was evident that in this large animal model, I-BMSCs and Al-BMSCs manifested similarly strong osteogenic potential, as significantly more new bone was formed in the Al-BMSC and I-BMSC groups than otherwise (...Continue Reading

References

Feb 24, 2001·The New England Journal of Medicine·R QuartoM Marcacci
Apr 4, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Songtao Shi, Stan Gronthos
Mar 5, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Takehiro MatsubaraYukio Kato
May 12, 2006·Periodontology 2000·Stan GronthosSongtao Shi
Sep 9, 2006·Clinical Oral Implants Research·Christian ClausenHenrik Nielsen
Oct 26, 2006·Journal of Dental Research·J J MaoS Shi
Jul 11, 2007·Journal of Cellular Physiology·Arnold I Caplan
Apr 25, 2008·Biomaterials·Gert J MeijerClemens A van Blitterswijk
May 22, 2008·Journal of Tissue Engineering and Regenerative Medicine·Stefan BajadaNureddin Ashammakhi
Nov 3, 2009·Periodontology 2000·Ni-Hung LinP Mark Bartold
Jul 14, 2010·Journal of Clinical Periodontology·Takanori IwataIsao Ishikawa
Jul 19, 2011·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Arash KhojastehMark Stevens
Aug 30, 2011·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Bu-Kyu LeeSe-Hoon Oh
Jan 10, 2012·Clinical Oral Implants Research·Igor Iuco Castro-SilvaJosé Mauro Granjeiro
Feb 9, 2012·International Journal of Dentistry·Toshiyuki YoshidaIsao Ishikawa
Apr 18, 2012·Periodontology 2000·Kim HynesP Mark Bartold
Jun 15, 2012·Biomaterials·Fa-Ming ChenQing Yu
Jul 11, 2012·Cell Transplantation·Darnell KaiglerWilliam V Giannobile

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Methods Mentioned

BETA
biopsies
chips
biopsy
flow cytometry
Scanning Electron Microscopy
fluorescence microscopy

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