PMID: 39111Jun 1, 1979

Comparison of ionic selectivity of batrachotoxin-activated channels with different tetrodotoxin dissociation constants

The Journal of General Physiology
L Y HuangG Ehrenstein

Abstract

The purpose of these experiments is to test whether the differences between normal and tetrodotoxin-resistant Na+ channels reside in the selectivity filter. To do this, we have compared the selectivity of batrachotoxin-activated channels for alkali cations, organic cations, and nonelectrolytes in two neuroblastoma clonal cell lines: N18, which has normal tetrodotoxin (TTX) sensitivity, and C9, which is relatively TTX-resistant. We have also studied the effect of H+ on Na+ permeability and on the interaction between TTX and its receptor site in both cell lines. There is no qualitative difference between the two cell lines in any of these properties. In both cell lines the batrachotoxin-activated Na+ channels have a selectivity sequence of Tl+ greater than Na+ greater than K+, guanidinium greater than Rb+ greater than Cs+, methylamine. Also, in both cell lines H+ blocks Na+ channels with a pKa of 5.5 and inhibits the action of TTX with the same pKa. These observations indicate that the selectivity filters of the Na+ channels in C9 and N18 do not differ significantly despite the 100-fold difference in TTX-affinity. Our selectivity studies of batrachotoxin-activated Na+ channels for both cell lines suggest that these toxin-activate...Continue Reading

References

Jun 4, 1971·Science·E X AlbuquerqueB Witkop
May 1, 1971·Acta Physiologica Scandinavica·P Redfern, S Thesleff
Jun 6, 1973·Nature: New Biology·J B Harris, M W Marshall
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·W A Catterall, M Nirenberg
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R HendersonG Strichartz
Jun 1, 1973·The Journal of General Physiology·A M Woodhull
Jun 1, 1972·The Journal of General Physiology·B Hille
Jan 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J MinnaM Nirenberg
Dec 1, 1971·The Journal of General Physiology·B Hille
Jul 1, 1971·The Journal of General Physiology·T NarahashiT Deguchi
Jul 1, 1967·Toxicon : Official Journal of the International Society on Toxinology·C Y Kao, F A Fuhrman
May 1, 1964·The Journal of General Physiology·T NARAHASHIW R SCOTT

Citations

Aug 1, 1990·Pflügers Archiv : European journal of physiology·U LönnendonkerR Stämpfli
Jul 1, 1984·Pflügers Archiv : European journal of physiology·H P VijverbergM Lazdunski
Jan 1, 1985·The Journal of Membrane Biology·L C McKinneyT Begenisich
Jan 1, 1985·Progress in Biophysics and Molecular Biology·B I Khodorov
Jul 1, 1987·Biophysical Journal·M F SheetsH A Fozzard
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·L Y HuangG Ehrenstein
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R P HartshorneM Montal
Feb 10, 2011·The Journal of Biological Chemistry·Yuzhe DuKe Dong
Jan 1, 1986·The Journal of General Physiology·M D LeibowitzB Hille
Mar 1, 1987·The Journal of General Physiology·S S Garber, C Miller
Jun 1, 1987·The Journal of General Physiology·W N GreenO S Andersen
Mar 1, 1991·The Journal of General Physiology·A M CorreaF Bezanilla
Jan 1, 1986·Annals of the New York Academy of Sciences·G StrichartzC H Bay
Jan 1, 1986·Annals of the New York Academy of Sciences·R P HartshorneM Montal
Oct 13, 2017·The Journal of General Physiology·Jon T Sack
Jan 1, 1985·The Journal of Membrane Biology·J A Talvenheimo
Jan 1, 1986·Membrane Biochemistry·A Uehara, E Moczydlowski

Related Concepts

Batrachotoxins
Cations
Cell Membrane Permeability
Clone Cells
Formamides
Hydrogen-Ion Concentration
Ion Channel
Neuroblastoma
Tetrodotoxin
Carmol

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