Comparison of Iron-Binding Ability Between Thr70-NapA and Ser70-NapA of Helicobacter pylori

Helicobacter
Weiran ShanRuiguang Ge

Abstract

The neutrophil-activating protein (NapA) of Helicobacter pylori (H. pylori), with DNA-binding and iron seizing properties, is a fundamental virulence factor involved in H. pylori-related diseases. Compared with Ser70-NapA strain, Thr70-NapA strain is more intimately correlated with iron-deficiency anemia. To investigate whether two types of proteins differ in iron-binding ability, mutated Thr70-NapA and Ser70-NapA strains were established. Isothermal titration calorimetry (ITC) method was conducted to measure the binding between the NapA protein and Fe(2+) . The structural changes of NapA protein were also tested during iron interaction by fast protein liquid chromatography (FPLC) and circular dichroism (CD) methods. DNA-binding assay was performed for evaluate the affinity of both mutated and wild types of NapA with DNA. Mutated Thr70-NapA had higher iron-binding ability than wild Ser70-NapA. The structural stability of Thr70-NapA was disrupted and became more active along with the rising concentration of Fe(2+) , whereas no similar association was observed between Ser70-NapA and Fe(2+) level. When the iron/protein molar ratio ranged from 10 to 20, both Ser70-NapA and Thr70-NapA displayed weaker DNA-binding ability. Thr70-NapA...Continue Reading

References

Jul 25, 1997·The Journal of Biological Chemistry·S TenebergK A Karlsson
Oct 23, 1997·Clinical Microbiology Reviews·B E DunnM J Blaser
Feb 19, 2002·Alimentary Pharmacology & Therapeutics·M F Go
Feb 22, 2002·European Journal of Immunology·Pasqualina MontemurroCesare Montecucco
Mar 14, 2002·International Journal of Medical Microbiology : IJMM·William G DundonCesare Montecucco
Oct 9, 2002·Journal of Molecular Biology·Giuseppe ZanottiCesare Montecucco
Jun 30, 2004·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Emilia ChianconeSimonetta Stefanini
Jul 10, 2004·Trends in Molecular Medicine·Mario M D'EliosGianfranco Del Prete
Mar 18, 2006·The Journal of Clinical Investigation·Amedeo AmedeiMarina de Bernard
Aug 31, 2006·Journal of the American Chemical Society·Ruiguang GeHongzhe Sun
Oct 13, 2006·Infection and Immunity·Ge WangRobert J Maier
May 25, 2007·FEMS Immunology and Medical Microbiology·Mario Milco D'EliosMarina de Bernard
Mar 31, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ghalia KhoderChristo Atanassov
Jul 25, 2009·Expert Review of Vaccines·Giuseppe Del GiudiceRino Rappuoli
Jul 17, 2010·Biochemistry. Biokhimii︠a︡·Meng-Chun ChiLong-Liu Lin
Feb 3, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Osamu TsurutaSatoshi Fujii

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Citations

Jul 14, 2020·Pediatrics International : Official Journal of the Japan Pediatric Society·Seiichi KatoUNKNOWN Japanese Society for Pediatric Gastroenterology, Hepatology and Nutrition

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