Comparison of isocratic retention models for hydrophilic interaction liquid chromatographic separation of native and fluorescently labeled oligosaccharides

Journal of Chromatography. a
Petr ČeslaJan Fischer

Abstract

In this work, we have investigated retention of maltooligosaccharides and their fluorescent derivatives in hydrophilic interaction liquid chromatography using four different stationary phases. The non-derivatized maltooligosaccharides (maltose to maltoheptaose) and their derivatives with 2-aminobenzoic acid, 2-aminobenzamide, 2-aminopyridine and 8-aminonaphthalene-1,3,6-trisulfonic acid were analyzed on silica gel, aminopropyl silica, amide (carbamoyl-bonded silica) and ZIC-HILIC zwitterionic sulfobetain bonded phase. The partitioning of the analytes between the bulk mobile phase and adsorbed water-rich layer, polar and ionic interactions of analytes with stationary phase have been evaluated and compared. The effects of the mobile phase additives (0.1% (v/v) of acetic acid and ammonium acetate in concentration range 5-30 mmol L(-1)) on retention were described. The suitability of different models for prediction of retention was tested including linear solvent strength model, quadratic model, mixed-mode model, and empirical Neue-Kuss model. The mixed-mode model was extended to the parameter describing the contribution of monomeric glucose unit to the retention of non-derivatized and derivatized maltooligosaccharides, which was u...Continue Reading

References

Nov 4, 2008·Mass Spectrometry Reviews·Manfred WuhrerAndré M Deelder
Dec 23, 2008·Journal of Proteome Research·David C A NevilleTerry D Butters
May 7, 2010·Journal of Chromatography. a·Uwe Dieter Neue, Hans-Joachim Kuss
Mar 29, 2011·Journal of Chromatography. a·Andreas E KaratapanisConstantine D Stalikas
Jul 9, 2011·Journal of Chromatography. a·Yong Guo, Sheetal Gaiki
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Jul 10, 2013·Carbohydrate Research·Qing FuXinmiao Liang

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Citations

Oct 14, 2017·Journal of Separation Science·Bob W J PirokPeter J Schoenmakers
Dec 29, 2020·Journal of Chromatography. a·Mimi J den UijlBob W J Pirok
Oct 16, 2020·Journal of Separation Science·Mimi J den UijlMaarten R van Bommel
Jan 24, 2021·Journal of Chromatography. a·Alexander KensertDeirdre Cabooter
Oct 22, 2020·Analytical Chemistry·Paul R HaddadRoman Szücs

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