Comparison of keratinocyte proliferation in diabetic and non-diabetic inflamed gingiva

Journal of Periodontology
Gökhan AçikgozSükrü Ozdamar

Abstract

Keratinocytes are chiefly cells of the epidermis but also constitute 90% of the gingival cells. The molecular mechanisms of proliferative activity in keratinization whereby diabetes alters periodontal physiology have not been elucidated. In this study, we aimed to investigate the role of gingival keratinocytes in hyperglycemic subjects by examining their mitotic activities. We tested 30 patients with periodontitis, of whom 15 were type II diabetics and the remainder systemically healthy. Biopsies were obtained from the bottom of the deepest pocket in each subject by reverse beveled incision. Formalin-fixed and paraffin-embedded specimens were then processed for periodic acid-Schiff (PAS)-diastase histochemistry and proliferating cell nuclear antigen (PCNA) (P10). Immunohistochemical studies were employed to determine the presence of PCNA and were used to detect the proliferating potential of keratinocytes needed in synthesizing DNA. The expression of PCNA was evaluated using an immunoperoxidase technique and PC10 monoclonal antibody to PCNA. Mitotic index was calculated from basal cells. Statistical analysis employed the chi-square test. No significant difference between the diabetic and non-diabetic patients was found in the m...Continue Reading

References

Feb 1, 1979·Journal of Oral Pathology·W J Hume, C S Potten
Jan 1, 1979·Journal of Periodontology·H T Nghiem, S S Stahl
Dec 1, 1992·International Journal of Oral and Maxillofacial Surgery·T TsujiF Shinozaki
Sep 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H K Wolf, K L Dittrich
Dec 1, 1992·Histopathology·D McCormick, P A Hall
Nov 1, 1991·Journal of Periodontal Research·I C MackenzieE B Lane
Mar 1, 1990·Journal of Clinical Pathology·P A Hall, D A Levison
Apr 2, 1987·Nature·R BravoH Macdonald-Bravo
Oct 1, 1969·Journal of Periodontology·N J BarakatN C Choukas
Jan 4, 1998·Journal of Periodontology·O M CarroC W Leone
Apr 1, 1997·International Dental Journal·R C Page, J D Beck
Jan 1, 1997·Vascular Medicine·D E McMillan
Feb 1, 1997·Periodontology 2000·H E Schroeder, M A Listgarten
Oct 22, 2002·Lancet·Mark A Marinella

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Citations

Jul 24, 2008·Special Care in Dentistry : Official Publication of the American Association of Hospital Dentists, the Academy of Dentistry for the Handicapped, and the American Society for Geriatric Dentistry·Andrei BaraschGregg H Gilbert
Nov 18, 2005·Lancet·Vincent Falanga
Dec 19, 2019·Periodontology 2000·Dana T GravesYingming Yang
Mar 15, 2019·Stem Cell Research & Therapy·Jolanta GoreckaAlan Dardik
Dec 11, 2020·Journal of Diabetes Investigation·Yanfen LiFuhua Yan

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