Comparison of laser-induced dissociation and high-energy collision-induced dissociation using matrix-assisted laser desorption/ionization tandem time-of-flight (MALDI-TOF/TOF) for peptide and protein identification

Rapid Communications in Mass Spectrometry : RCM
Marcus MachtSören-Oliver Deininger

Abstract

The fragmentation of peptides under laser-induced dissociation (LID) as well as high-energy collision-induced dissociation (CID) conditions has been investigated. The effect of the different fragmentation mechanisms on the formation of specific fragment ion types and the usability of the resulting spectra, e.g. for high-throughput protein identification, has been evaluated. Also, basic investigations on the influence of the matrix, as well as laser fluence, on the fragment ion formation and the consequences in the spectral appearance are discussed. The preconditions for obtaining 'pure' CID spectra on matrix-assisted laser desorption/ionization tandem time-of-flight (MALDI-TOF/TOF) instruments are evaluated and discussed as well as the differences between LID and CID in the resulting fragment ion types. While containing a wealth of information due to additional fragment ions in comparison with LID, CID spectra are significantly more complex than LID spectra and, due to different fragmentation patterns, the CID spectra are of limited use for protein identification, even under optimized parameter settings, due to significantly lower scores for the individual spectra. Conditions for optimal results regarding protein identification...Continue Reading

Citations

Nov 19, 2011·Journal of the American Society for Mass Spectrometry·Mathieu DupréChristine Enjalbal
Nov 21, 2008·Journal of the American Society for Mass Spectrometry·Chul YooMinhui Ma
Nov 9, 2004·Journal of Mass Spectrometry : JMS
Dec 20, 2008·Journal of Mass Spectrometry : JMS·Nawar ShenarChristine Enjalbal
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Feb 11, 2012·Mass Spectrometry Reviews·Pilar Perez Hurtado, Peter B O'Connor
Sep 8, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cristian PeptuJaroslav Mosnáček
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Jul 11, 2006·Journal of Proteome Research·Jae-Won YangGert Lubec
Nov 16, 2005·Analytical Chemistry·Plamen A DemirevJeffrey S Lin

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