Comparison of Leishmania OligoC-TesT PCR with conventional and real-time PCR for Diagnosis of canine Leishmania infection.

Journal of Clinical Microbiology
Connor CarsonO Courtenay

Abstract

There is a need for standardization and simplification of the existing methods for molecular detection of Leishmania infantum in the canine reservoir host. The commercially available OligoC-TesT kit incorporates standardized PCR reagents with rapid oligochromatographic dipstick detection of PCR products and is highly sensitive for use in humans but not yet independently validated for use in dogs. Here we compare the sensitivity of OligoC-TesT with those of nested kinetoplast DNA (kDNA) PCR, nested internal transcribed spacer 1 (ITS-1) PCR, and a PCR-hybridization protocol, using longitudinal naturally infected canine bone marrow samples whose parasite burdens were measured by real-time quantitative PCR (qPCR). The sensitivity of OligoC-TesT for infected dogs was 70% (95% confidence interval [CI], 63 to 78%), similar to that of kDNA PCR (72%; 95% CI, 65 to 80%; P = 0.69) but significantly greater than those of PCR-hybridization (61%; 95% CI, 53 to 69%; P = 0.007) and ITS-1 nested PCR (54%; 95% CI, 45 to 62%; P < 0.001); real-time qPCR had the highest sensitivity (91%; 95% CI, 85 to 95%; P < 0.001). OligoC-TesT sensitivity was greater for polysymptomatic and oligosymptomatic dogs than for asymptomatic dogs (93%, 74%, and 61%, res...Continue Reading

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Citations

Jan 18, 2013·PLoS Neglected Tropical Diseases·Rupert J QuinnellOrin Courtenay
Jan 15, 2014·PLoS Neglected Tropical Diseases·Orin CourtenayRupert J Quinnell
Jun 4, 2016·Journal of Microbiological Methods·Dimitra K ToubanakiEvdokia Karagouni
Oct 1, 2015·Revista Do Instituto De Medicina Tropical De São Paulo·Rodrigo StaggemeierSabrina Esteves de Matos Almeida
Aug 5, 2015·The American Journal of Tropical Medicine and Hygiene·Alejandro Castellanos-GonzalezBruno L Travi
Sep 17, 2014·The American Journal of Tropical Medicine and Hygiene·Fadime ErogluIsmail Soner Koltas
Jan 13, 2018·PLoS Neglected Tropical Diseases·Bruno L TraviGuadalupe Miró
Oct 20, 2020·Parasitology·Joyce Carvalho PereiraVanete Thomaz Soccol
Aug 23, 2018·Trends in Parasitology·Malcolm S DuthieOrin Courtenay

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