Comparison of linear and branched peptide forms (MAPs) in the induction of T helper responses to point-mutated ras immunogens

Cellular Immunology
M E SchottS I Abrams

Abstract

The utility of multiple antigenic peptides (MAPs) for the induction of antibody and cellular immune responses in animal models has been demonstrated for a variety of peptide epitopes involved in human disease. However, little is known about immune responses to MAPs constructed with antigenic tumor epitopes, nor has peptide specificity in branched forms been addressed. A potentially important advantage of the MAP system over linear peptide immunogens for clinical applications is elimination of the need for a protein carrier with its associated toxicity and immunogenicity. Here, we examined cellular immune responses following in vivo administration of MAPs incorporating a 13-mer T helper epitope from point-mutated ras p21 (ras V12) and compared the potency of the responses to that of the linear peptide. The Gly --> Val mutation in position 12, which is associated with a range of human carcinomas, represents a useful system for evaluating the specificity of the immune response. In initial studies with the point-mutated linear peptide epitope, optimal in vitro proliferation responses were obtained following sc administration of the peptide in a squalane-containing adjuvant formulation. Comparative immunization studies using point-m...Continue Reading

Citations

Jun 8, 2013·Amino Acids·Alessandro GoriGiorgio Colombo
Feb 25, 2009·Expert Opinion on Biological Therapy·Chiara FalcianiLuisa Bracci
Jul 5, 2005·Expert Opinion on Investigational Drugs·J SchlomJ W Hodge
Jun 20, 2002·Journal of Biotechnology·Kristen Sadler, James P Tam

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